安神补脑液对睡眠剥夺模型大鼠学习记忆及脑源性神经营养因子表达的影响  

Effect of Anshenbunao syrup on learning and memory and BDNF expression in hippocampus of sleep deprivation rats

作　　者：魏海峰[1] 叶翠飞[1] 李春阳[1] 丁月霞[1] 赵宁[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室教育部神经变性病重点实验室北京脑老化重点实验室,北京100053

出　　处：《中国临床药理学与治疗学》2006年第11期1230-1233,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(№30340090);国家重点基础研究发展规划(973)项目(№G2003CB517104);北京市自然科学基金项目(№7032013;7992010)

摘　　要：目的:研究安神补脑液对睡眠剥夺大鼠的学习记忆能力影响,并从脑源性神经营养因子(BDNF)角度探讨其机理。方法:SD大鼠,随机分成正常对照组、正常对照+安神补脑液小剂量组及模型+蒸馏水组及模型+安神补脑液大、小剂量组。灌胃给予安神补脑液2周后,用多站台水环境法进行睡眠剥夺造模4d,用避暗法测试其学习记忆能力,并取海马组织用Westernblot法测定BDNF表达量。结果:与正常对照组比较,睡眠剥夺大鼠避暗测试潜伏期显著减短,表明出现学习记忆能力障碍;大剂量安神补脑液给药能显著延长模型大鼠的潜伏期,表明该药可有效减轻大鼠睡眠剥夺后的学习记忆障碍。安神补脑液也能延长正常大鼠避暗实验的潜伏期。在睡眠剥夺大鼠海马组织BDNF表达量显著降低,而用安神补脑液干预后模型大鼠BDNF表达量增加,在大剂量组出现显著性差异。结论:安神补脑液能提高睡眠剥夺鼠的学习记忆能力,其机理之一可能与提高海马BDNF含量有关。AIM： To study the effects of An-Shen-Bu-Nao syrup （AS） on the learning and memory ability and BDNF expression in the hippocampus of sleep deprivation （SD） rats. METHODS： 75 rats were divided into groups of control, control ＋ AS （0.02 ml· kg^-1 ）, SD ＋ distillated water （DW）, SD ＋ AS （0.02 ml· kg^-1 ）, and SD ＋ AS （0.06 ml· kg^-1 ）. All rats were fed with AS or DW for 2 weeks, and they were subjected to sleep deprivation for 4 days, then the passive avoidance was tested for learning and memory. Also the BDNF expression in the hippocampus was tested with western blot after sacririce. RESULTS： Compared with the control rats, SD rats had the significantly reduced latency in passive avoidance test, showing the decreased learning and memory ability. SD combined with AS （0.06 ml· kg^-1 ） rats showed significantly longer latency than the SD rats （ P 〈 0.05）, indicating the better learning and memory ability than the sleep deprivation rats; BDNF expression was significanfly higher in the rats of SD combined with AS （0.06ml· kg^-1 ） than the SD rats. CONCLUSION： An-Shen-Bu-Nao Syrup can effectively improve the learning and memory ability of sleep deprivation rats, and the enhancement of BDNF expression may contribute to this effect.

关 键 词：安神补脑液 睡眠剥夺 学习记忆:脑源性 神经营养因子 海马 大鼠 

分 类 号：R965.2[医药卫生—药理学]