甘草不同品系种子特征特性比较与遗传多样性分析  

Seed Property Comparison and Genetic Diversity Analysis among Different Lines of Glycyrrhiza uralensis

作　　者：张爱华[1] 雷锋杰[1] 陈长宝[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《吉林农业大学学报》2006年第6期644-647,651,共5页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20020501)

摘　　要：试验研究了8个甘草(Glycyrrhiza uralensis)不同品系种子的特征、特性及其遗传多样性。结果表明:甘草不同品系间在种子大小、发芽率、发芽势等方面存在显著差异(P<0.05)。进一步利用ISSR标记对甘草8个品系的遗传多样性进行分析,发现甘草不同品系间存在明显的遗传多样性差异,共扩增出42条不同的产物,其中多态性片段34条(占80.95%),并发现了2条特异性带。通过计算遗传相似性系数,建立了UPGMA聚类图。Seed properties and genetic diversity among different lines of Glycyrrhiza uralensis were studied. The results showed that shape and gemfinating rate were different among 8 lines. Genetic diversity among 8 lines was investigated with ISSR technique. Amplified results showed a total record of 42 bands, among which 34 （80.95%） were polymorphic. By calculating genetic similarity coefficient, UPGMA clustering map was made. ISSR is valuable to breeding selection for Glycyrrhiza uralensis.

关 键 词：甘草 种子特性 简单重复序列间区 遗传多样性 

分 类 号：S567.71[农业科学—中草药栽培]