温度对中华鲟精子、卵子短期保存的影响  

Effect of Temperature on Short-Term Preservation of Chinese Sturgeon Sperm and Egg

作　　者：郑跃平[1] 刘鉴毅[2] 谢从新[1] 危起伟[2] 杨德国[2] 陈细华[2] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]农业部淡水鱼类种质资源与生物技术重点开放实验室中国水产科学研究院长江水产研究所

出　　处：《吉林农业大学学报》2006年第6期682-686,共5页Journal of Jilin Agricultural University

基　　金：国家公益研究专项基金(2000DIB50177;2004DIB3J099);国家基础性工作重点基金(2002DEA1004);国家自然科学基金重大项目(30490231)

摘　　要：研究了中华鲟精子和卵子在低温（8，12℃）、中温（16，20℃）和高温（24，28℃）条件下短期保存的效果。结果发现：精子保存时间随着保存温度的升高逐渐缩短，8℃下可保存146h以上，而28℃下只能保存32h。低温下保存精子的活力强于高温下保存的精子，表现为快速运动时间和总活动时间较长；卵子适合在中温（16，20℃）下保存，高温或者低温对卵子的保存都不利，28℃下只能保存4-8h，8℃下只能保存4-10h，而在16℃和20℃下可保存18-20h。The effect of low temperature （8, 12 ℃ ）, medium temperature （ 16, 20 ℃ ） and high temperature （24, 28 ℃ ） on short-term preservation of Chinese sturgeon sperm and egg was studied for the first time. The result showed： Along with the temperature rising, the preservation time of sperm reduced. Sperm could be preserved under 8 ℃ for above 146 h, compared with only 32 h under 28 ℃. The motility of sperm preserved under low temperature （8, 12 ℃ ） was stronger than the others, and had longer quick-movementtime and total-movementtime. The egg was suitable to be preserved under medium temperature （ 16, 20 ℃ ）, while high temperature or low temperature was all disadvantageous for egg preservation. Egg could only be preserved for 4-8 h under 28℃, only 4 - 10 h under 8℃, however, 18 - 20 h under 16℃ and 20℃.

关 键 词：中华鲟 精子 卵子 温度 短期保存 

分 类 号：S931.1[农业科学—渔业资源]