livin靶向RNA干扰质粒的鉴定  

Identification of livin-targeting RNA-interfering plasmid

作　　者：张玉萍[1] 饶冬梅[1] 张春斌[1] 刘爽[1] 吕冬霞[1] 罗佳滨[1] 吕学诜[1] 

机构地区：[1]佳木斯大学基础医学院生物教研室,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2006年第6期25-26,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省教育厅科学技术研究项目(编号:11511399);黑龙江省研究生创新科研资金项目(编号:YJSCX2005-113HLJ)

摘　　要：目的:验证liv in靶向RNA干扰重组质粒是否构建成功并成功转化入大肠杆菌。方法:从已经转化的大肠杆菌中提取质粒,并经限制性内切酶酶切、A grose电泳,测序。结论:酶切图谱分析,测序结果显示重组质粒已成功构建并转化入大肠杆菌。Objective： To verify whether the livin--targeting RNA -- interfering recombinant plasmids are successfully constructed and are transformed into E. coli. Methods：The plasmids were extracted from transformed E. coli, and then electrophoresis was performed with agrose gel after being digested by the restriction enzyme, and sequence analysis was made. Conclusion： The recombinant plasmids have been successfully constructed and transformed into E. coll.

关 键 词：LIVIN RNAI 质粒 

分 类 号：Q344[生物学—遗传学]