家蚕血细胞在体外培养过程中的变化  

Changes of Hemocytes From the Silkworm (Bombyx mori) in vitro Culture

作　　者：徐伟[1] 潘敏慧[1] 张金叶[1] 洪锡钧[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与生物技术学院农业部蚕桑学重点开放实验室,重庆400716

出　　处：《蚕业科学》2006年第4期588-591,共4页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB121000);国家自然科学基金项目(编号30471312);国家科技攻关专题(编号2005BA711A07)。

摘　　要：为建立家蚕血细胞系,以家蚕血淋巴为材料,对其进行了长达1年多的原代培养,并从形态学上进行了观察与分类。详细记录了培养过程中出现的多种细胞或组织的分化或转分化现象,发现有2种家蚕血细胞在体外培养条件下有一定的增殖能力,暗示家蚕血细胞体外建系具有可行性。In order to establish hemocyte line, the hemolymph from silkworm larvae had been primary cultured about one year. Based on morphological observation and taxonomy, the differentation and transdifferentation of cells and tissues were recorded in details. It was found that two kinds of hemocytes had proliferation activity, which implied that it is likely to establish cell line from Bombyx moil hemocytes in vitro.

关 键 词：家蚕 血细胞 体外培养 分类 分化 

分 类 号：Q813.11[生物学—生物工程] S881[农业科学—特种经济动物饲养]