定喘汤加减方颗粒中麻黄和黄芩TLC的研究  

Study on TLC Identification of Herba Ephedrae and Radix scutellariae in Clinical Add-subtrace granule Preparation of dingchuantang

作　　者：黄江虹[1] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510120

出　　处：《国际医药卫生导报》2006年第24期78-79,共2页International Medicine and Health Guidance News

摘　　要：目的研究该颗粒中麻黄和黄芩薄层色谱鉴别的方法。方法分别取麻黄和黄芩药材、该颗粒剂及阴性制剂的提取液,前者在硅胶G薄层板上以氯仿-甲醇-氨水(10:4:0.30)为展开剂,以茚三酮试液显色;后者黄芩的鉴别:硅胶G薄层板,正丁醇-冰乙酸-水(7:1:2)为展开剂,1%三氯化铁乙醇溶液为显色剂;结果在供试品、对照品及对照药材色谱相应的位置上,显相同颜色的斑点;阴性对照液在相应的位置上未见斑点。结论此方法经多次比较,专属性强,为进一步研究该颗粒的质量标准提供有力的科学依据。Objective to study the TLC identification of Herba Ephedrae and Radix scutellariae in Clinical Add-subtrace granule Preparation of dingchuantang. Methods the distill of Herba Ephedrae and Radix scutellariae, this granule and the blankness were developed in Silica gel G plate with chloroform-methanol-ammonia water （10：4：0,30）as development reagent and triketohydrindene hydrate as color developing reagent in the former one. The identification of Radix scutellariae was silica gel G plate, n-butanol-glacial acetic acid-water（7：1：2） as the development reagent, chloride ferric（1%） as color developing reagent. Results There are identical points in the same position among the sample, reference substance and reference crude herb. And there are no points in the blankness. Conclusions the method has a good specificity compared by many times. And it can provide the significant prove for the study of quality control.

关 键 词：薄层色谱法 麻黄 黄芩 

分 类 号：R286[医药卫生—中药学]