钾维普对未成熟心肌细胞缺血-再灌注损伤保护作用  

The Protective Effect of Kalium-Verapamil-Propranolol Cardioplegic Solution on Cultured Immature Cardiac Myocytes Injured by Ischemia-Reperfusion

作　　者：吴枝娟[1] 王瑞幸[1] 王雅锋[1] 方秋娟[1] 

机构地区：[1]福建医科大学生理学与病理生理学系,福州350004

出　　处：《齐齐哈尔医学院学报》2006年第11期1285-1287,共3页Journal of Qiqihar Medical University

基　　金：福建省教育厅科研基金资助项目(K04051)

摘　　要：目的研究钾维普停搏液对未成熟心肌细胞缺血-再灌注损伤的保护作用。方法以乳鼠未成熟心肌细胞为材料建立缺血-再灌注损伤模型,从心肌细胞搏动频率、细胞生存率、心肌酶学等方面观察钾维普停搏液对心肌细胞缺血-再灌注损伤的保护作用。结果与模型组比较,钾维普组肌酸激酶、丙二醛释放量明显降低,心肌细胞搏动频率、细胞生存率及超氧化物歧化酶活性明显升高。结论钾维普停搏液显著减轻未成熟心肌细胞缺血-再灌注损伤,效果优于高钾液或维拉帕米、普萘洛尔的单独作用。Objective To study the protective effect of kalium--verapamil propranolol（.KVP） cardioplegia on cultured immature rat cardiac myocytes iniured by isehemia--reperfusion. Methods The ischemia--reperfuslon injury model of cultured neonatal rat cardiac myoeytes was developed. The spontaneous beating, surviving rate, the content of Creatinkinase, Superoxide Dismutase, Malondialdehyde in supernatant after ischemia--reperfusion were detected. Results Compared with the measurements taken from ischemia--reperfusion model group, the CK and MDA in supernatant decreased sharply; the sponta- neous beating,surviving rate of cardiac myoeytes and the activity of SOD increased highly, Conclusions The KVP eardioplegia can obviously lessen the injury of ischemia--reperfusion in immature cardiac myocytes. The KVP cardioplegia is superior to hyperkalium cardioplegia,verapamil and propranolol.

关 键 词：心脏停搏液 心肌保护 缺血-再灌注损伤 维拉帕米 普萘洛尔 

分 类 号：R285.5[医药卫生—中药学]