掘氏疫霉抗土菌灵突变体的诱导及其特性研究  

Induction and Characteristics of Mutants of Phytophthora drechsleri Resistant to Etridiazole

作　　者：朱志峰[1] 潘洪玉[2] 张浩[1] 姜辉[3] 袁善奎[3] 严清平[3] 

机构地区：[1]吉林农业大学资源与环境学院,吉林长春130118 [2]吉林大学植物科学学院,吉林长春130062 [3]农业部农药检定所生物技术研究测试中心,北京100026

出　　处：《农药学学报》2006年第4期323-326,共4页Chinese Journal of Pesticide Science

摘　　要：掘氏疫霉Phytophthora drechsleri是引起黄瓜疫病的主要病原菌。采用紫外线诱导获得了20株掘氏疫霉抗土菌灵的突变体，并对其生物学特性进行了研究。结果表明，掘氏疫霉对土菌灵的抗性突变频率高于对甲霜灵的抗性突变频率，但其抗性水平低于抗甲霜灵突变体，在1．3—4．3倍之间；掘氏疫霉对土菌灵的抗性在继代培养和常温保存过程中稳定，突变体的生长速率与亲本敏感菌株相比发生了不同程度的变化；此外，掘氏疫霉抗土菌灵突变体对甲霜灵和烯酰吗啉无交互抗性。Twenty etfidiazole-resistant mutants of Phytophthora drechsleri were obtained by ultraviolet irradiation, and some of their biological characteristics were studied in vitro, The results showed that frequency of mutation in P. drechsleri that resistant to etridiazole was higher than that resistant to metalaxyl, while etridiazole-resistant level was much lower than that of metalaxyl-resistance, the resistance factor（RF） for etridiazole was ranged from 1.32 to 4.31. The resistance to etridiazole was stable in the process of continuous transfer cultivation and long conservation under room temperature. As compared to their parental sensitive isolate, the growth rates of mutants changed more or less. In addition,there was no cross-resistance between etridiazole and metalaxyl or dimethomorph.

关 键 词：掘氏疫霉 土菌灵 抗性突变体 特性 

分 类 号：S481.4[农业科学—农药学]