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作 者:胡燕[1] 王开运[1] 许学明[1] 夏晓明 杨焕青[1] 王刚
机构地区:[1]山东农业大学植物保护学院农药科学系,山东泰安271018 [2]山东省农业科学研究院棉花研究中心,山东济南250000 [3]中国烟叶公司,北京100055
出 处:《农药学学报》2006年第4期339-343,共5页Chinese Journal of Pesticide Science
基 金:国家烟草专卖局重点项目(国烟科[2003]579号)
摘 要:从山东和云南烟区采集分离了20个烟草黑胫病菌phytophthora parasitica vat.nicotianae菌株,采用菌丝生长速率法测定了烯酰吗啉对这些菌株的毒力。结果表明:20个田间菌株的EC50值在0.60-1.28μg/mL之间,与实验室保存的敏感菌株(S)的EC50值(0.32μg/mL)相比,毒力倍数在1.87—4.00之间.均属于敏感菌株。烯酰吗啉对烟草黑胫病菌孢子囊的产生有明显的抑制作用,8.00μg/mL的烯酰吗啉对孢子囊产生的抑制率为100%。通过烯酰吗啉对烟草黑胫病菌继代培养物的毒力比较证明,烟草黑胫病菌对烯酰吗啉存在抗性风险。The inhibitory effect of dimethomorph on 20 strains of Phytophthora parasitica var. nicotianae,which isolated from Shandong and Yunnan province, were determined by using mycelium growth rate method. The results showed that EC50 values of 20 strains were ranged from 0.60 to 1.28 μg/mL, Compared with of the sensitive strain EC50 value, the inhibitory factors were ranged from 1.87 to 4.00, which indicated that the 20 strains were sensitive to dimethomorph. Dimethomorph could evidently inhibit the formation of sporangia. The inhibitory rates against the sporangia formation of P. parasitica was 100% by 8. 00 μg/mL dimethomorph. The resistance risk of P. parasitica to dimethomorph was proved by testing on inhibitory activity of dimethomorph to mycelial growth of the sub-culture fungus.
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