家犬粪便中的细粒棘球绦虫DNA PCR检测方法的建立  

A method established for detectintg Echinococcus granulosus DNA from dog faeces using PCR

作　　者：张亚楼[1] 温浩[1] 马旭东[1] 苗玉清[1] 陈雪鸿[1] 姜涛[1] 张春[1] 刘辉[1] 

机构地区：[1]新疆医科大学一附院新疆维吾尔自治区包虫病基础医学重点实验室,新疆乌鲁木齐830054

出　　处：《中国病原生物学杂志》2006年第5期363-365,共3页Journal of Pathogen Biology

基　　金：新疆维吾尔自治区重点实验室开放课题基金项目(No.2005-XJDX0202-02)

摘　　要：目的建立检测家犬粪便中细粒棘球绦虫DNA的PCR方法,为开展流行病学监测提供检测手段。方法犬粪经液氮反复冻溶后提取DNA,PCR扩增编码细粒棘球绦虫12s rDNA检测该靶DNA片段(255 bp)。结果10只细粒棘球绦虫感染犬,8只(体内成虫数>80条)阳性,2只(体内成虫数分别为4条和5条)阴性。4只未感染家犬粪样及其他3种绦虫DNA样本均为阴性。结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。Objective To establish a way suitable for PCR amplification and identify Echinococcus granulosus DNA in dog faeces and provide a tool for epidemlologleal purpose. Methods DNA isolation from dog excrement was performed according to procedure involving lysis in liquid nitrogen. A 255 bp DNA fragment of mitochondrial cytochrome 12s rDNA was amplified from dog faeces by PCR. Results The PCR test could check 8 dogs with E. granulosus adult worm burden over 80 worms, But for 2 dogs with worm burden only 4 and 5, the PCR result showed negative. And 4 dogs without Echinococcus spp. could not be amplified by the PCR-based method. Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed.

关 键 词：细粒棘球绦虫 PCR 特异性 灵敏性 终宿主 

分 类 号：R383.33[医药卫生—医学寄生虫学]