海南龙血树的组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Dracaena cambodiana

作　　者：李克烈[1] 李志英 徐立[1] 马千全[1] 罗联忠[1] 黄碧兰[1] 陈伟[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《热带农业科学》2006年第5期21-23,共3页Chinese Journal of Tropical Agriculture

基　　金：国家科技基础条件平台重点项目"热带作物种质资源收集;整理;保存和保护关键技术和标准研究(2003DIA3N022)"。

摘　　要：用种子作外植体,建立了海南龙血树(DracaenacambodianaPierreexGagnepain)的组织培养和再生体系。结果表明:海南龙血树种子诱导愈伤组织的最佳培养基为MS+6-BA3mg/L+NAA0.3mg/L,诱导率达80%;附加6-BA2mg/L+NAA0.2mg/L的MS培养基有利于芽的分化和增殖,培养60d左右,每个外植体分化形成的再生小植株数达8～20个;在1/2MS+NAA0.5mg/L的培养基中诱导生根效果最好。The seeds of Dracaena cambodiana Pierre ex Gagnepain were used as explants to establish a tissue culture and regeneration system. The best medium for callus induction from seeds was found to be the MS ＋ 3 mg/L 6-BA ＋ 0.3 mg/L NAA. The induction frequency was 80%. The MS supplemented with 2 mg/L 6-BA and 0.2 mg/L NAA was favorable to differentiation and proliferation of buds, and each explant regenerated 8-20 plantlets about 60 days of culture. The optimum root-inducing medium was 1/2 MS supplemented with NAA 0.5 mg/L.

关 键 词：海南龙血树 组织培养 快速繁殖 

分 类 号：S567.19[农业科学—中草药栽培] Q831[农业科学—作物学]