凤尾兰的组织培养  

Tissue Culture in Yucca gloriosa Linnaeus

作　　者：庞仪[1] 张月雄[1] 罗远华[1] 莫饶[1] 

机构地区：[1]华南热带农业大学农学院,海南儋州571737

出　　处：《热带农业科学》2006年第5期24-27,共4页Chinese Journal of Tropical Agriculture

基　　金：中国热带农业科学院;华南热带农业大学科技基金项目"几种热带药用植物离体快繁的研究"。

摘　　要：以凤尾兰(YuccagloriosaLinnaeus)的茎尖、幼嫩茎段及嫩叶为外植体进行组织培养。结果表明以嫩叶为外植体的愈伤组织诱导率最高,由茎段诱导的愈伤组织继代增殖最好,由茎尖诱导的愈伤组织诱导不定芽表现最佳。筛选出最佳初代培养基MS+6-BA3.0mg/L+NAA0.2mg/L;愈伤组织增殖培养基MS+6-BA8.0mg/L+NAA0.1mg/L;不定芽诱导培养基为MS+6-BA5.0mg/L+KT1.0mg/L;不定芽增殖培养基为MS+6-BA4.0mg/L+NAA0.05mg/L+椰子水100mL/L;生根培养基为1/2MS+NAA0.2mg/L。Using the stem tip and tender stem shoot and leaf as the explants, the tissue culture of Yucca gloriosa Linnaeus was studied. The callus-inducing rate from the tender leaf was the highest and the induced calli from the tender stem were the best for subculture, while the induced calli from the stem tip produced best adventitious buds. The optimum media for each culture stages were： MS ＋ 6-BA 3.0 mg/L ＋ NAA 0.2 mg/L ＋ sucrose 30 g/L for formation of callus; MS ＋ 6-BA 8.0 mg/L ＋ NAA 0.1 mg/L ＋ 30 g/L for callus subculture for multiplication; MS ＋ 6-BA 5.0 mg/L ＋ KT 1.0 mg/L ＋ sucrose 30 g/L for inducing adventitious buds; MS ＋ 6-BA 4.0 mg/L ＋ NAA 0.05 mg/L ＋ CW 100 mL/L＋ sucrose 30 g/L for buds multiplication; 1/2 MS ＋ NAA 0.2 mg/L ＋ sucrose 30 g/L for rooting.

关 键 词：凤尾兰 组织培养 愈伤组织 不定芽 

分 类 号：Q813.1[生物学—生物工程]