FSK88对UMR106细胞中cbfa1基因表达的影响  

Effect of FSK88 on cbfa1 Gene Expression in Rat Osteosarcoma UMR106 Cell

作　　者：耿硕[1] 孙波[1] 王敬泽[1] 

机构地区：[1]中国科学院动物研究所生物膜与膜工程国家重点实验室

出　　处：《河北师范大学学报（自然科学版）》2006年第6期702-705,共4页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金资助项目(30171071)

摘　　要：以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化.3次重复实验显示:与对照组相比,经25,50,100μmol/L 3种浓度FSK88药液处理的细胞内cbfa1基因表达量分别增加35.5%,64.5%,124.0%.其中用100μmol/L的FSK88处理细胞后,cbfa1基因表达量有显著的上调(P<0.01),并且上调作用强于10μmol/L的维甲酸药液.证明FSK88能够通过直接或间接增强UMR106细胞中cbfa1基因的表达,从而促进肿瘤细胞向正常细胞逆转分化.Expression of cbfal gene in rat osteosarcoma UMR106 cell was detected by RT-PCR after the 72 h＇s treatment of FSK88 liquid with different mol concentration （25.50.100μmol/L）. Retinoic Acid （RA. 10μmol/L） and mixed liquid of FSK88 and RA （1 ： 1）. The results of three repeated experiments demonstrated that：compared with the control group. cbfa1 gene expression of the cells treated with 25.50. 100μmol/L FSK88 had an increase of 35.5 % .64.5 %. 124.0 % respectively. Especially after treatment of 100μmol/L FSK88 liquid. UMR106 cell showed an significantly enhancive cbfal gene expression （P 〈 0.01） .and the increase rate was bigger than the cells treated with 10μmol/L Retinoic Acid. It was proved that FSK88 could induce cancer cells to differentiation through enhancing the expression of cbfal gene directly or indirectly

关 键 词：FSK88 cbfa1基因 成骨肉瘤 RT-PCR 

分 类 号：R966[医药卫生—药理学]