新疆棉花黄萎病菌病原种群监测研究  

Population monitoring study of Verticillium dahliae in cotton in Xinjiang

作　　者：张莉[1] 段维军[1] 李国英[1] 杨之为[2] 

机构地区：[1]新疆兵团绿洲生态农业重点实验室,新疆石河子832003 [2]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2006年第11期189-193,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：新疆兵团棉花枯黄萎病监测与防治研究项目;新疆兵团绿洲生态农业重点实验室项目

摘　　要：采用致病性测定、营养亲和群测定及PCR特异性扩增3种方法,对新疆棉区采集的35个棉花黄萎病菌代表性菌系进行致病型鉴定。结果表明,新疆棉花黄萎病菌存在强、中、弱3种不同的致病类型,其中以中等致病类型居多。利用营养亲和群方法将新疆棉花黄萎病菌分为2个亲和群,其中1个亲和群中的所有菌系均与标准落叶型菌系相亲和,另1个亲和群中的所有菌系均与标准非落叶型菌系相亲和。利用棉花黄萎病菌落叶型特异引物进行PCR特异性扩增,有3个菌系扩增出550bp的落叶型黄萎病菌特异性片段。用营养亲和群及PCR特异性扩增均进一步证实目前新疆存在落叶型黄萎病菌菌系。The pathogenicity identification of 35 representative strains of Verticillium dahliae collected from the cotton-planted region in Xinjiang was studied using the methods of pathogenicity identification, vegetative compatibility group and PCR specific amplification. The results show： （1） the strains oi Verticillium dahliae in Xinjiang can be separated into three groups,namely strong,middle and weak. Among them, most of the strains belong to the middle group. （2） the strains of Verticillium dahliae in Xinjiang can be separated into two vegetative compatibility groups. All strains in one vegetative compatibility group can be fused with standard defoliating pathotype and all strains in the other vegetative compatibility group can be fused with standard nondefoliating pathotype. This result makes sure the presence of the defoliating pathotype strains of VerticiUium dahliae in cotton. （3） The result of PCR amplification using the specific primer of defoliating pathotype of Verticilliura dahliae in cotton shows that the specific fragment （550 bp） of defoliating pathotype of Verticillium dahliae can be produced from three strains,which proves the presence of the defoliating pathotype strains of Verticillium dahliae in cotton in Xinjiang at present under the molecular level.

关 键 词：棉花黄萎病菌 致病性 营养亲和性 PCR扩增 新疆棉区 

分 类 号：S435.621.24[农业科学—农业昆虫与害虫防治]