甲醛在体外对HEK293细胞Rab26基因复制的影响  

The Influence of Formaldehyde on the Replication of Rab26 Gene in HEK293 Cell in Vitro

作　　者：姜英[1] 袁均林[1] 徐钱[1] 吴凯[1] 吴扬[1] 谭宏亮[1] 杨旭[1] 丁书茂[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,湖北武汉430079

出　　处：《化学与生物工程》2006年第11期45-48,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(30570799)

摘　　要：以HEK293细胞为材料,经不同浓度的甲醛(≥250μmol.L-1)染毒后,检测其DPC的含量,同时对染毒后HEK293基因组中的Rab26基因进行PCR扩增。结果表明:随着甲醛浓度的升高,DPC的含量显著升高;Rab26基因的扩增量随甲醛浓度的升高、DPC含量的升高而降低。说明高浓度甲醛能够诱导DPC产生,同时说明Rab26基因形成了DPC,因此,扩增量下调。In this study HEK293 cell was used as material and treated with HCHO（≥250μmol·L^-1） with different concentration. The result showed that accompany elevating the. concentration of HCHO the amount of DPC significantly enhanced（P〈0.01）, at the meantime it was found HCHO and the amount of DPC enhanced, the amount of replication of coupled with the concentration of Rab26 was reduced by PCR.

关 键 词：甲醛 DNA-蛋白质交联(DPC) Rab26 小的GTP结合蛋白(LMWGs) 

分 类 号：R994.6[医药卫生—毒理学]