人重组GM－CSF／HAS－D3嵌合蛋白（GM－HSA）在大肠杆菌中的表达及其产物稳定性的研究  

Overexpression and Stability of Recombinant Human Chimeric Protein rhGM-CSF/HSA-D3 (GM-HSA)in E.coli

作　　者：朱振齐[1] 刘进[1] 马大龙[1] 张颖妹[1] 吴跃红 

机构地区：[1]北京医科大学免疫系分子免疫室

出　　处：《生物化学杂志》1996年第3期284-288,共5页

摘　　要：将人粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）和人血清白蛋白第三功能区（ＨＳＡ－Ｄ３）的基因串联后，在Ｅ．ｃｏｌｉ中获高效表达，表达量占菌体蛋白的３２．６％。利用ＴＦ－１体外细胞活性测定表明，ＧＭ－ＨＳＡ的活性单位为１．０４×１０＾６Ｕ／ｍｇ，虽然其比活性低于ＧＭ－ＣＳＦ，但比后者具有更高的体外热稳定性和储藏稳定性。A recombinant vector pMT-GM-HSA expressing the chimeric protein which contained the entire human GM-CSF and domain 3 of HSA had been constructed. The chimeric protein was expressed up to 32.6% of total bacterial protein. In vitro bioactivity analysis with TF-1 cell line showed that the GM-HSA protein expressed a specific stimulating activity of 1.04×10_￣6 U/mg on the proliferation of TF-1 cells. In vitro the GM-HSA showed much higher thermostability and storage stability than GM-CSF.

关 键 词：嵌合蛋白 粒细胞 巨噬细胞 集落刺激因子 稳定性 

分 类 号：Q513.02[生物学—生物化学]