神经节苷脂GM_3对肌质网Ca^（2＋）－ATP酶活力的调节  

The Effect of Ganglioside GM_3 on the Enzyme Activity of Sarcoplasmic Reticulum Ca￣(2+)-ATPase

作　　者：王丽华[1] 崔肇春[1] 屠亚平[1] 杨福愉 

机构地区：[1]大连医科大学生化教研室,中国科学院生物物理所生物大分子国家重点实验室

出　　处：《生物化学杂志》1996年第3期308-312,共5页

基　　金：生物大分子国家重点实验室基金

摘　　要：研究了神经节苷脂ＧＭ_３参入肌质网膜后Ｃａ^（２＋）－ＡＴＰ酶活力的变化，结果表明：ＧＭ_３参入肌质网膜后，对肌质网Ｃａ^（２＋）ＡＴＰ酶活性（ＡＴＰ水解活力与转运活力）有明显的激活作用．当参入的ＧＭ_３浓度为８μｍｏｌ／Ｌ、参入时间为１２０ｍｉｎ、温度为３０℃时，对Ｃａ^（２＋）－ＡＴＰ酶的激活作用最大．Ganglioside GM_3 from canine erythrocytes was incubated with rabbit sarcoplasmic reticulum (SR) vesicles to observe its effect on SR Ca￣(2+)-ATPase. The results showed that GM_3 could incorporate into the membrane of SR and significantly activated SR Ca￣(2+)-ATPase (including ATP hydrolysis activity and Ca￣(2+)-uptake activity). Such an activation was dependent on GM_3 concentration,and incubation temperature and time. The optimum activation was observed when 8μmol/L GM_3 was incubated with SR vesicles for 120 min at 30℃.

关 键 词：神经节苷脂GM3 肌质肉 腺苷三磷酸酶 钙 

分 类 号：Q546.02[生物学—生物化学]