卤虫脱壳卵的液氮冷冻保存研究  

CRYOPRESERVATION OF DECAPSULATED ARTEMIASP. EMBRYOS

作　　者：刘向宇[1] 陆仁后[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《水生生物学报》1996年第3期248-256,共9页Acta Hydrobiologica Sinica

摘　　要：作者以甘油、ＤＭＳＯ、葡萄糖和蔗糖为冷冻保护剂对卤虫脱壳卵进行了液氮冷冻保存研究．各保护剂对短期吸水发育的胚胎有一定的冷冻保护作用，在本实验条件范围内，高浓度保护剂较低浓度保护剂好，双重保护剂（甘油＋ＤＭＳＯ、葡萄糖＋蔗糖）冷冻保护作用较单一保护剂效果好。饱和ＮａＣｌ溶液对各发育时期的胚胎均有较好的冷冻保护作用，但对后期胚胎的冷冻保护作用较弱。作者认为胚胎内冰晶的形成是卤虫脱壳卵冷冻致死的主要原因。冷冻保护剂对卤虫脱壳卵的冷冻保护作用可能与它们的脱水作用有关。The decapsulated Anemia sp.embryos at their blastula stage were successfullycryopreserved in liquid nitrogen with various concentrations of glycerol,DMSO,glucose andsucrose as single cryoprotectants. Higher cryoprotectant concentrations were better than thelowers Dual cryoprotectants (glycerol+DMSO, glucose+sucrose)had stronger cryoprotectionthan single-type cryoprotection. Saturated NaCl solution showed a powerful cryoprotpetionfor various developmental stages of Anemia sp. embryos,although a weaker cryoprotCctionwas observed for later stage.Intraembryoic ice crystal formation was the main reason causing the death of the embryos.The cryoprotection of various cryoprotectants is possibly related to their dehydration effect.

关 键 词：甲壳纲 卤虫 脱壳卵 冷冻保存 液氮 

分 类 号：Q959.223[生物学—动物学]