复方多康宁(Co—DKL)对铅引起的发育大鼠海马CA1区长时程增强效应(LTP)损伤的修复作用  

Co—DKL restores the lead-induced impairment of the long-term potentiation in the hippocampal CA1 region of developing rats

作　　者：李秀芳[1] 阮迪云[2] 马建元[1] 钟桂生[2] 白莉莉[1] 胡徽东[1] 耿仁义[1] 罗乐[2] 

机构地区：[1]中国人民解放军105医院,合肥230031 [2]中国科学技术大学生命科学学院,合肥230027

出　　处：《微量元素与健康研究》2006年第6期6-9,共4页Studies of Trace Elements and Health

摘　　要：目的：慢性铅暴露可以引起儿童学习记忆和认知功能的损伤。突触可塑性被认为是学习记忆的神经生物学基础，长时程增强（long-term potentiation，LTP）是突触可塑性的一种重要形式。已有的研究表明慢性铅暴露损伤了大鼠海马CA1区和齿状回LTP的诱导和表达过程。本实验应用离体脑片电生理技术研究了复方多康宁（Co-DKL）对慢性铅暴露引起的发育中大鼠海马突触可塑性损伤的修复效应。方法：新生Wistar幼鼠出生后经母乳摄入铅和在铅暴露的同时往食物中添加3种不同剂量的Co-DKL[0．8、1．6、3．2mg／（kg·d）].在30～32d的大鼠海马CA1区记录EPSP。结果：慢性铅暴露损伤了CA1区LTP的幅度；3种不同剂量的Co-DKL对铅处理组LTP的幅度有不同程度的提高，其中低剂量Co-DKL几乎完全恢复了铅引起的CA1区LTP的损伤。而高剂量Cxr-DKL的修复作用较小且稍有一点毒副作用。合适剂量的Co-DKL能有效逆转铅造成的神经系统损伤，可能具有一定的促智作用。结论：低剂量的Co-DKL（正常剂量的2倍）对铅引起的损伤有显著的修复作用，对对照组大鼠有一定的促智作用，且没有副作用；中剂量（正常剂量的4倍）有一定的修复作用，但高剂量的修复作用较小，有一定的毒副作用。Aim：Lead （Pb） contributes to a variety of learning, memory and cognitive dysfunction for children. Long-term potentiation （LTP）, an important form of synaptic plasticity, is believed to underlie learning and memory. Previous studies have demonstrated that Pb impairs LTP of CA1 and Dentate gyrus（DG） regins in developing rat hippocampus. This study was carried out to investigate whether Pb-induced impairment of synaptic plasticity could be reversed by the Co-DKL. Methods： Neonatal Wistar rats were exposed to Pb from parturition by drinking of 0.2 % AcPb solution or a diet supplemented with three different dosages Co - DKL[ 0.8,1.6,3.2mg/ （kg- d） ] with 0.2 % AcPb . Exdtatory postsynaptic potentials （EPSPs） were recorded in the hippocampal CA1 region of rats （30 -- 32 days）. Results：The results showed that Pb reduced the amplitude of LTP significantly. In Pb- exposed rats, three dosages of Co- DKL had a different effect on the amplitudes of rat hippocampal LTP and low dosage Co - DKL was almost restored the Pb - induced impairments, while effective role of the middle and high dosages Co - DKL was less than low dosage and high dosage Co- DKL had a little toxicity. Conclude： Co- DKL could dose - dependently restore Pb - induced impairment of synaptic plasticity, low dosage Co- DKL was almost restored the Pb - induced impairments and might have an effective role for improving intelligence, while effective role of the middle and high dosages Co - DKL was less than low dosage and high dosage Co - DKL had a little toxicity.

关 键 词：铅 Co-DKL 长时程增强 双脉冲易化 

分 类 号：R284[医药卫生—中药学] O614.433[医药卫生—中医学]