缬沙坦对心肌病大鼠肾脏及心肌中ACE2 mRNA、ACE mRNA表达的影响  

Effect of valsartan on expression of angiotensin-converting enzyme 2 mRNA and ACE mRNA in the kidney and myocardium in cardiomyopathic rats

作　　者：王琼英[1] 余静[1] 杨成悌[1] 张缤[1] 

机构地区：[1]兰州大学第二医院心内科,730030

出　　处：《国际心血管病杂志》2006年第6期419-422,共4页International Journal of Cardiovascular Disease

基　　金：甘肃省科学事业费项目(QS041-C33-17);甘肃省自然科学基金项目(ZS031-A25-054-E)

摘　　要：目的：探讨阿霉素诱导的心肌病大鼠肾脏及心肌组织中血管紧张素转换酶2(ACE2)mRNA、血管紧张素转换酶(ACE)mRNA的表达及缬沙坦对其的影响。方法：30只Wistar大鼠随机分为3组：正常对照组即生理盐水组(n=10)；生理盐水+阿霉素组(n=10)；阿霉素+缬沙坦组(n=10)。RT-PCR法检测肾、心肌组织中ACE2 mRNA、ACE mRNA的表达,光镜及透射电镜观察心肌病理变化。结果：阿霉素组大鼠较正常对照组大鼠肾脏及心肌组织中ACE2 mRNA(3．35±0．39对2．21±0．18,P=0．000；2．50±0．29对1．25±0．13,P=0．000)、ACE mRNA (2．31±0．38对1．19±0．12,P=0．000；1．77±0．09对0．84±0．11,P=0．000)的表达均明显增高,缬沙坦治疗组大鼠肾脏及心肌组织中ACE2 mRNA(2．65±0．34对3．35±0．39,P=0．001；1．72±0．32对2．50±0．29,P=0．000)、ACE mRNA (1．68±0．15对2．31±0．38,P=0．004；1．21±0．10对1．77±0．09,P=0．000)的表达较阿霉素组明显降低。结论：阿霉素诱导的心肌病大鼠肾脏及心肌组织中ACE2 mRNA、ACE mRNA表达水平均显著高于正常大鼠；缬沙坦对于阿霉素诱导的心肌病大鼠肾脏及心肌具有保护作用。Objective： To determine the levels of mRNA expression of ACE2 and ACE on doxorubicin（DOX）-induced cardiomyopathy in rats, and to explore its changes with valsartan. Methods：Twenty adult Wistar rats were given DOX（ 2.5 mg· kg^-1. p. twice weekly）. Four weeks after the administration of DOX, 10 rats were treated with valsartan（400 mg · kg^-1 , p.o. daily） for 6 weeks, and 10 rats received saline were used as a control group. The levels of mRNA expression of ACE2 and ACE were examined by RT-PCR. Histology was per,formed in DOX rats after 10 weeks in both groups. Results;ACE2 mRNA（kidney, 3.35±0.39 vs 2.21 ± 0.18,P=0. 000;heart, 2.50± 0.29 vs 1.25±0.13,P=0.000 and ACEmRNA（kidney, 2. 31±0. 38 vs 1.19±0.12,P=0.000; heart, 1.77 ± 0.09 vs 0.84 ± 0. 11 ,P=0. 000） was significantly increased in the DOX-induced kidney and heart as compared to that in the controls. The expression of ACE2 and ACE mRNA in DOX-rats was decseased with valsartan treatment （ACE2： kidney, 2.65 ± 0. 34 vs 3. 35 ± 0, 39,P= 0. 001; heart, 1.72 ± 0.32 vs 2. 50 ± 0. 29 , P= 0. 000 ; ACE; kidney, 1.68 ± 0. 15 vs 2.31 ± 0.38, P = 0. 004;heart, 1.21 ± 0.10 vs 1.77 ± 0.09, P 0.000）. Conclusions： These data indicate that ACE2 is up regulated in DOX-indueed cardiomyopathy. Valsartan could protect the kidney and heart during cardiomyopathy of rat.

关 键 词：心肌病 大鼠 血管紧张素转换酶2 基因表达 缬沙坦 

分 类 号：R542.21[医药卫生—心血管疾病]