乙醇-超声法在浮游植物叶绿素a含量测定中的应用  

Application of ethanol-sonication methods to the determination of phytoplankton chlorophyll-a content

作　　者：杨洪芳[1] 丁峰元[2] 陈德辉[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国水产科学研究院东海水产研究所,上海200090

出　　处：《海洋渔业》2006年第4期309-313,共5页Marine Fisheries

基　　金：农业部海洋与河口渔业重点开放实验室开放课题(开1-04-03)

摘　　要：叶绿素测定是生物监测及水质评价中估算藻类生物量的重要指标。以超声波破碎藻细胞,以热乙醇为萃取剂测定浮游植物叶绿素a的含量,并与常规的丙酮-研磨法进行比较对照,结果证实该方法是可行的。其提取时间仅为10m in,因此尤适于快速检测。The determination of chlorophyll is a key index in monitoring water quality. Algal cells were broken up with ultrasonic and chlorophyll-a was extracted with hot ethanol from phytoplankton. This method was compared with a regular method by acetone-rubbing. It was proved that ethanol-sonication could be used normally in the determination of chlorophyll-a, especially in an emergency, because chlorophyll-a could be extracted in only ten minutes.

关 键 词：浮游植物 叶绿素A 乙醇 超声法 丙酮 

分 类 号：Q945.11[生物学—植物学]