钙对NaCl胁迫下百合花粉萌发、脯氨酸含量和SOD活性的影响  

Effects of Exogenous Ca^(2+) on Proline Content and SOD Activity of Germinated Pollens under Salt Stress

作　　者：丁彬[1] 岳晓翔[1] 彭建云[1] 王宝山[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《菏泽学院学报》2006年第5期98-102,142,共6页Journal of Heze University

基　　金：国家自然科学基金资助项目(30270793);山东省自然科学基金重点资助项目(Z2004D03)

摘　　要：以百合花粉为实验材料,采用花粉液体培养法探讨了植物信号物质钙在NaCl抑制百合花粉萌发中的作用.结果表明:NaCl对百合花粉萌发有抑制作用,抑制程度随培养基中NaCl浓度的增大而加强,甚至完全抑制花粉萌发,NaCl显著抑制百合花粉萌发的浓度为25 mmol/L,此时花粉萌发率下降到32.3%,仅为对照的52.5%.Ca(NO3)2能够部分缓解NaCl对百合花粉萌发的抑制作用,其缓解作用的浓度为1.5~3mmol/L,其中以2 mmol/L对NaCl胁迫的缓解效果最佳,花粉萌发率达到最大,为50.7%.超过此浓度范围后,缓解作用下降,甚至出现抑制作用.NaCl胁迫条件下,外源钙显著降低盐胁迫下萌发花粉的脯氨酸含量而增加SOD活性.L ilium pollens were used to study effects of exogenous Ca^2＋ on pollen germination under NaCl stress. The results showed that NaCl stresss significantly inhibited pollen germination. The inhibition increased with increase of NaCl concentration, and even pollen germination was absolutely suppressed by higher NaCl. When NaCl was at 25 mmol/L, the pollen germination was significantly reduced, and the germination rate decreased to 32.3 % and 52.5 % of that of control. Exogenous Ca（NO3）2 partly alleviated the inhibition of pollen germination in a range of 1.5 - 3 mmol/L and the optimal concentration was at 2 mmol/L, at which the germinatlon rate was 50.7 % Higher Ca^2＋ than 3 mmol/L inhibited the pollen germination. Exogenous Ca^2＋ markedly reduced the praline content, but increased SOD activity during pollen germination under salt stress.

关 键 词：百合 花粉萌发 CA^2+ NACL胁迫 脯氨酸 SOD 

分 类 号：Q945.78[生物学—植物学]