Polyaniline-graphite composite film glucose oxidase electrode  

作　　者：周海晖 陈宏 陈金华 旷亚非 

机构地区：[1]College of Chemistry and Chemical Engineering, Hunan University

出　　处：《Journal of Central South University of Technology》2006年第6期653-657,共5页中南工业大学学报（英文版）

基　　金：Projects(50473022, 20673036) supported by the National Natural Science Foundation of China; project(2005) supported by the State Key Laboratory of Chemo/Biosensing and Chemometrics of China; project(2006FJ4100) supported by the Science Technology Project of Hunan Province; project(2006) supported by the Postdoctor Foundation of Hunan University

摘　　要：A novel polyaniline-graphite composite film glucose oxidase （PGCF GOD） electrode was developed. The PGCF was synthesized by cyclic voitammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition ofpH 5.5, 0.8 V and 65℃.A novel polyaniline-graphite composite film glucose oxidase (PGCF GOD) electrode was developed. The PGCF was synthesized by cyclic voltammetry method in 0.5 mol/L H2SO4 solution containing 1 g/L graphite powder and 0.2 mol/L aniline. The PGCF GOD electrode was prepared by doping GOD into the composite film. The morphology of the PGCF and the response property of the PGCF GOD electrode were investigated by scanning electron microscopy and electrochemical measurement, respectively. The results show that the PGCF has a porous and netty structure and the PGCF GOD electrode has excellent response property such as high sensitivity and short response time. Influences of pH value, temperature, glucose concentration and potential on the response current of the electrode were also discussed. The sensor has a maximum steady-state current density of 357.17 μA/cm2 and an apparent Michaelis-Menten constant of 16.57 mmol/L. The maximum current response of the enzyme electrode occurs under the condition of pH 5.5, 0.8 V and 65 ℃.

关 键 词：POLYANILINE GRAPHITE composite film glucose oxidase BIOSENSOR 

分 类 号：O646.54[理学—物理化学]