青蒿组织培养体系建立的研究  

Study on the Building of the Artemisia Annua L.Cultivation System

作　　者：穆胜玉[1] 白志川[1] 宋孝霞[1] 

机构地区：[1]西南大学,重庆400716

出　　处：《重庆科技学院学报（自然科学版）》2006年第4期21-24,35,共5页Journal of Chongqing University of Science and Technology：Natural Sciences Edition

摘　　要：为建立青蒿品种改良及其快速繁殖技术体系,以青蒿试管苗子叶和胚轴、嫩叶、田间栽培青蒿的花序为外植体,研究其离体培养及试管苗再生途径。结果表明,以青蒿花序为外植体,以MS+6-BA 0.5mg/l+NAA 1.0mg/l为愈伤组织诱导培养基,愈伤组织在MS+6-BA 4.0mg/l+NAA 0.05mg/l培养基上分化,以MS+6-BA 2.0mg/l+6-KT 2.0mg/l为扩繁培养基,分化苗转到MS+NAA 1.0mg/l培养基上生根。可以用较低成本,较短时间,比较简便地得到大量青蒿再生试管苗。In order to investigate the callus induction and the regeneration of the plant of Artemisia annua L., the cotyledon,hypokotyl, lamina and anthotaxy are used as explants. The result shows that the anthotaxy is placed on MS＋6-BA 0.5mg/l＋NAA 1.0mg/l to induce callus, which differentiats on MS＋ 6-BA 4.0mg/l＋NAA 0.05mg/l.The croud buds is propogated on ＋6-BA 2.0mg/l＋6-KT 2.0mg/l, finaUy, croud buds can root on MS＋NAA1.0mg/l. Hence, we can get plenty of regeneration test-tube buds easily with low cost and less time.

关 键 词：青蒿 青蒿素 花序 愈伤组织 

分 类 号：R931.2[医药卫生—生药学]