载入海藻糖红细胞冷冻干燥保存的实验研究  

Beneficial effects of intracellular trehalose on red blood cells by freeze drying

作　　者：何晖[1] 刘宝林[1] 华泽钊[1] 李川[1] 沈敏 陈颖 

机构地区：[1]上海理工大学动力工程学院,上海200093 [2]上海安图医院血液检验科,上海200093

出　　处：《上海理工大学学报》2006年第6期571-576,共6页Journal of University of Shanghai For Science and Technology

基　　金：国家自然科学基金资助项目(50376040);国家自然科学基金重点资助项目(50436030);上海市重点学科建设资助项目(T0503);上海市引进海外高层次留学人员专项资金资助项目

摘　　要：探讨载入海藻糖对红细胞冷冻干燥保存的效果.利用硫酸蒽酮比色法测量细胞内海藻糖的含量,对载入海藻糖的红细胞进行了冷冻干燥保存.结果表明,细胞载入海藻糖的最高浓度为35mmol/L,经冷冻干燥后的细胞回收率与血红蛋白回收率提高非常明显,分别为冷冻干燥前的59.78±4.51%和57.49±3.69%;渗透脆性与新鲜红细胞无显著差异.载入海藻糖对红细胞的冷冻干燥保存效果有密切作用.Effects of intracellular trehalose on red blood cells by freeze drying are investigated. Trehalose is loaded into fresh red blood cells after 7 hours＇ incubation at 37℃ in various concentration trehalose solutions （400,600,800,1 000 mmol/L）. The results indicate that the intracellular trehalose concentration is increased with the increasing concentration of extracellular trehalose solution. The maximum concentration of intracellular trehalose is 35 mmol/L in this study. Red blood cells loaded with trehalose are lyophilized. Results show that the viability （59.78 ± 4.51） % and the recovery of hemoglobin （57.49 ± 3.69） % of incubated group increase remarkably compared to those of control group. Osmotic fragility data show that trehalose exerts osmotic protection on RBCs during freezedrying.

关 键 词：红细胞 冷冻干燥 载入海藻糖 

分 类 号：R619.6[医药卫生—外科学]