银屑病患者淋巴细胞与中性粒细胞中趋化因子受体CXCR3 mRNA的表达  

Expression of CXCR3 mRNA in peripheral blood lymphocytes and neutrophils in patients with psoriasis

作　　者：杨桂兰[1] 陈志强[2] 郑家润[2] 李新宇[2] 陈沄[2] 徐兰芳[2] 高纪伟[2] 唐美育[2] 

机构地区：[1]兰州军区兰州总医院皮肤科,甘肃兰州730050 [2]中国医学科学院中国协和医科大学皮肤病研究所,江苏南京210042

出　　处：《第四军医大学学报》2006年第22期2065-2067,共3页Journal of the Fourth Military Medical University

基　　金：卫生部科技专项基金(981041)

摘　　要：目的:了解银屑病患者外周血淋巴细胞与中性粒细胞中CXC型趋化因子受体CXCR3 mRNA的表达水平及其与银屑病皮损面积和严重程度指数(PASI)之间的关系.方法:应用逆转录-聚合酶链反应(RT-PCR)法检测进行期斑块型银屑病患者33例及其中16例患者治疗后外周血淋巴细胞及中性粒细胞中CXCR3 mRNA的表达水平,设30例健康人作为正常对照,并将检测结果与PASI进行了相关性分析.结果:斑块型银屑病患者外周血淋巴细胞中CXCR3 mRNA表达水平为1.12±0.81,明显高于健康对照组(0.28±0.28,P<0.01)与治疗后患者(0.57±0.61,P<0.05),并与PASI之间呈明显正相关(r=0.516,P<0.05);而中性粒细胞中CXCR3 mRNA表达水平为1.72±1.68,与健康对照组(1.27±1.17)及同一组患者治疗后(1.27±1.01)相比均无明显差异(P>0.05).结论:CXCR3可能主要通过活化与趋化外周血淋巴细胞而参与了银屑病的发病机制.AIM： To investigate the expression of chemokine receptor CXCR3 mRNA in peripheral blood lymphocytes and neutrophils in patients with progressive plaque psoriasis and its correlation with Psoriasis Area and Severity Index （PASI）. METHODS： Reverse transcriptase-polymerase chain reaction （RT-PCR） was applied to semi-quantitatively analyze CXCR3 mRNA expression in peripheral blood lymphocytes and neutrophils from 33 psoriatic cases, 16 cases with about 70% remission of the 33 patients after treatment and 30 healthy controls. The correlation between CXCR3 mRNA expression and PASI was evaluated. RESULTS： CXCR3 mRNA level in lymphocytes from psoriatic patients was 1. 12 ± 0.81, markedly higher than that in normal controls （0. 28 ±0.28, P 〈0. 01 ）and treated patients （0. 57 ± 0.61, P 〈 0.05 ）. Furthermore, a positive correlation was noticed between CXCR3 mRNA expression and PASI （ r = 0. 516, P 〈 0.05）. However, there was no statistic significance in CXCR3 mRNA level in neutrophils among pretreated psoriatic patients （ 1.72 ± 1.68 ）, normal controls （ 1.27 ± 1.17, P 〉 0.05 ） and treated patients（ 1.27 ± 1.01, P 〉 0.05）. CONCLUSION ： The increased expression of CXCR3 in lymphoeytes may be associated with migration and aggregation of lymphoeytes in psoriatic lesion,suggesting that this chemokine receptor be involved in the pathogenesis of progressive plaque psoriasis.

关 键 词：银屑病 淋巴细胞 受体CXCR3 趋化因子 CXC 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]