Fmoc固相合成法合成黑色素细胞刺激素(MSH)的工艺研究  

Fmoc solid-phase synthesis of MSH peptide

作　　者：朱立国[1] 任付平[1] 陈五岭[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《第四军医大学学报》2006年第22期2100-2102,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究9-芴甲氧羰基(Fmoc)固相合成法合成黑色素细胞刺激素(MSH)工艺的步骤及其影响因素.方法:采用Fmoc保护α-氨基,以TBTU/HOBT/D IEA为缩合剂合成MSH.用色谱仪和质谱仪对合成的多肽进行纯化、鉴定.结果:多肽合成的得率可达63%,经反相高效液相色谱仪(RP-HPLC)对合成的多肽进行纯化后可得纯度为98.67%的目标肽.通过基质辅助激光解吸附电离飞行质谱仪(MALD I-MS)检测所合成多肽的分子质量为1663.88 ku,与其理论分子质量1664.08 ku相符.结论:该合成法具有快捷、简便、高效地合成多肽MSH的特点,适合大批量生产.AIM： To study the process and influencing factors of synthesizing the melanocyte stimulating hormone （MSH） peptide by Fmoc solid-phase synthesis. METHODS： With Fmoc to protect the α-amino acid, TBTU/HOBT/DIEA were used as coupling reagents to synthesize the peptide of MSH. The synthesized peptide was purified by the chromatograph and identified by mass spectrograph. RESULTS： The peptide yield was 63%. The synthesized peptide purity reached to 98.67% after being purified by RP-HPLC. The molecular mass of the synthesized peptide was 1663.88 ku by MALDI-MS and it was consistent with the theoretic molecular mass of 1664.08 ku. CONCLUTION ： This method of synthesis is rapid,simple and effective for the peptide MSH, and it＇s fit for the large-scale production.

关 键 词：固相合成 肽类 MSH 芴甲氧羰基 

分 类 号：R978.1[医药卫生—药品]