甘孜藏猪与合作猪SLA-DQA基因的群体遗传研究  

Research on population genetics of SLA.DQA gene in Hezuo pigs and Ganzhi pigs

作　　者：李华[1] 蒋岸岸[1,2] 于辉[1] 吕学斌[3] 李学伟[2] 赵海全[1] 

机构地区：[1]佛山大学生命科学学院,佛山528231 [2]四川农业大学动物科技学院,雅安625014 [3]四川省畜牧科学研究院,成都610061

出　　处：《高技术通讯》2006年第12期1294-1299,共6页Chinese High Technology Letters

基　　金：国家自然科学基金（30300246）资助项目.

摘　　要：对甘孜藏猪和合作猪SLA-DQA基因的第1和第2内含子部分序列以及完整的第2外显子进行了PCR-RFLP分析，结果表明：经EcoRⅠ酶切后，BB基因型频率（0．5537）分别高于AB型（0．3719）和从型（0．0744）；B为优势等位基因（0．7397）。经AluⅠ酶切后，MM基因型频率（0．4380）分别高于MN型（0．3637）和NN型（0．1983）；M为优势等位基因（0．6198）。2品种中AluⅠ酶切基因型在合作猪中未达到Hardy-Weinberg平衡，且群体间M、N的基因频率和NN基因型频率差异分别达极显著（P〈0．01）和显著水平（P〈0．05）。共鉴别了9种组合基因型，甘孜藏猪和合作猪分别为6和8种组合基因型，BB-MM、BBNN组合基因型频率在两群体间差异显著（P〈0．05）。两酶切位点均表现为中度多态，AluⅠ位点多态信息含量均高于EcoRⅠ位点，合作猪杂合性高于甘孜藏猪，两群体间的遗传距离为0．0389。The partial sequences of intron 1 and intron 2, and the complete sequence of exon 2 of SLA-DQA gene in Ganzhi Zang pigs （GZ） and Hezuo pigs （HZ） were analysed by PCR-RFLP. The results showed that the genotype frequency of BB （0.5537） at EcoR I-RFLP site was higher than that of AB （0.3719） and AA （0.0744）. B （0.7397） was the predominant allele. To the Alu I-RFEP site, the genotype frequency of MM （0.4380） was higher than that of MN （0.3637） and NN （0. 1983） in the two population pigs, and M （0.6198） was the predominant allele. The values of χ^2（CHITEST） showed that the genotypes at Alu I site in Hezuo pigs were not fit with Hardy-Weinberg equilibrium. There were significant differences for M, N allele frequency （ P 〈 0.01 ） and NN genotype frequency （ P 〈 0.05） between GZ and HZ at different levels. There were nine PCR-RFLP patterns of SLA-DQA gene in the two population pigs, six in GZ and eight in HZ, respectively. The genotype frequency of BBMM and BBNN was significantly different between the two population pigs （ P 〈 0.05）. Both sites were moderately polymorphic, but the polymorphism information content （PIC） at Alu I site was higher than that at EcoR I site, the heterozygosity was higher in HZ than that in GZ, and the Nei＇s unbiased genetic distance was 0.0389 between the two population pigs.

关 键 词：藏猪 SLA-DQA PCR-RFLP 遗传参数 

分 类 号：Q75[生物学—分子生物学]