二硫化碳对大鼠支持细胞凋亡及FasL表达的影响  

Apoptosis and gene FasL expression induced by carbon disuifide in rat steroli cells

作　　者：邓菁[1] 陈国元[1] 季佳佳[1] 谭皓[1] 王松[2] 虎凤仙[2] 柴莲花[2] 刘四海[2] 刘卫东[2] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室,武汉430030 [2]华中科技大学同济医学院公共卫生学院预防医学实验教学中心,武汉430030

出　　处：《中华劳动卫生职业病杂志》2006年第12期730-733,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(30471442)

摘　　要：目的探讨二硫化碳对离体培养支持细胞凋亡及FasL基因表达的影响。方法以0、0．36、0．72、1．44μmol/ml浓度的二硫化碳作用于离体培养的大鼠支持细胞24 h后,应用噻唑蓝(MTT)法检测支持细胞存活率,流式细胞术检测细胞凋亡及逆转录聚合酶链反应(RT-PCR)技术检测凋亡相关基因FasL的表达。结果二硫化碳处理后的支持细胞存活率随作用浓度的增加而下降,当≥1．44μmol/ml浓度时,细胞存活率(73．34%±1．39%)明显下降,与溶剂对照组(99．98%±5．48%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度升高,细胞凋亡率增加,当浓度≥1．44μmol/ml时,支持细胞凋亡率(7．93%±0．43%)明显升高,与溶剂对照组(3．63%±0．53%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度增加,凋亡相关基因FasL表达量亦明显增加。结论二硫化碳对离体培养支持细胞具有细胞毒性作用,可导致细胞凋亡率升高,凋亡相关基因FasL高表达。Objective To study apoptosis and gene FasL expression induced by carbon disulfide in steroh cells of male rats. Methods Steroli cells were exposed to different concentrations of CS2（0,0.36,0.72,1.44 μmol/ml） for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively. Results Steroli cell survival rate decreased as the concentration of CS2 increased. The survival rate（73.34% ± 1.39%） was significantly lower than the control group（99.98% ± 5.48% ） when the concentration of CS2≥ 1.44 μmol/ml（ P 〈 0.05）.Apoptosis rate increased as the CS2 concentration increased. Apoptosis rate（7.93 % ± 0.43 % ） was significantly higher when the concentration of CS2 ≥ 1.44 μmol/ml （ P 〈 0.05）. Expression level of the FasL significantly increased as the concentrations of CS2 （ P 〈 0.05）. Conclusion CS2 is cytotoxic to steroli cells. It could cause apoptosis of steroli cells.

关 键 词：二硫化碳 细胞凋亡 基因 

分 类 号：R114[医药卫生—卫生毒理学]