惊恐应激对大鼠下丘脑单胺类递质的影响及中药干预  

Effects of Fear Stress on the Contents of Monoamine Neurotransmitters in Hypothalamus of Rats and The Intervention of Chinese Medicine

作　　者：董秋安[1] 刘晓伟[1] 许丽[1] 顾靖[2] 丁慧波[1] 陈欣[1] 陈窕圆[1] 

机构地区：[1]南方医科大学中医药学院临床基础教研室,广东广州510515 [2]复旦大学上海医学院医学神经生物学国家重点试验室,上海200032

出　　处：《辽宁中医杂志》2006年第12期1652-1653,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30271567);广东省中医药管理局基金资助项目(02102074)

摘　　要：目的:观察惊恐应激大鼠下丘脑单胺类神经递质及其代谢产物的变化,以及补肾固涩方药桑螵蛸散对其干预作用。方法:根据Takash i方法建立惊恐应激模型,采用HPLC-ECD检测惊恐应激后大鼠下丘脑单胺类神经递质及其代谢产物的含量,并与正常组、桑螵散给药组进行对照。结果:惊恐模型组大鼠下丘脑E、5-HT的含量显著下降,与空白对照组比较,差异显著(P<0.01,P<0.05);桑螵散给药组大鼠下丘脑E及5-HT含量、5-HT/DA比值明显升高,与模型组相比有显著性差异(P<0.05);模型组与空白对照组大鼠NE/DA、5-HT/DA、5-HT/NE比值无显著性差异(P>0.05)。结论:惊恐应激可以导致大鼠脏腑气机紊乱,下丘脑中E、5-HT含量下降可能是其发病机制之一;桑螵蛸散能改善大鼠惊恐状态,与调节下丘脑中E、5-HT含量和5-HT/DA比值有关。Objective： To investigate the changes of monoamine neurotransmittcr and their metabolite in hypothalamus of model rats with fear stress and the effects of Sangpiaoxiao Decoction（SPXD） Methods ：To establish the fear stress model according to Takashi＇s method and determine the contents of monoamine neurotransmitters and their metabolites by HPLC - ECD. Compare fear group, SPXD group and normal group. Results ： The lower contents of E, 5 - HT had been observed in hypothalamus of model rats compared with - normal group（ P 〈 0.01, P 〈 0.05）. On the contrary, the contents of both rose significantly in SPXD group. The comparisons of NE/DA , 5 - HT/DA and 5 - HT/NE in fear group and normal group have no significant discrepancy（ P 〉 0.05 ） ,SPXD can regulate the 5 - HT/DA significantly （ P 〈 0. 05 ）. Conclusion ： The mechanism of Qi injured by fear sress is involved with the lower contents of E,5 -HT in hypothalamus,SPXD can improve the situation of fear by regulating the contents of E, 5 - HT and 5 - HT/ DA.

关 键 词：惊恐 应激 下丘脑 单胺类递质 中药 

分 类 号：R285.6[医药卫生—中药学]