以层柱黏土为载体固定辣根过氧化物酶  

Immobilization of horseradish peroxidase to pillared clay as carrier

作　　者：于少明[1] 程俊[1] 左鹏[1] 陈天虎[2] 史铁钧[1] 

机构地区：[1]合肥工业大学化学工程学院 [2]合肥工业大学资源与环境工程学院,安徽合肥230009

出　　处：《化工学报》2006年第12期3021-3024,共4页CIESC Journal

基　　金：合肥工业大学中青年科技创新群体培养计划资助~~

摘　　要：Aluminum pillared interlayered clay （Al-PILC） calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase （HRP） among several materials investigated.HRP could be immobilized on the Al-PILC via hydrogen bonding between functional groups of support and amino groups of the enzyme.The support and immobilized enzyme were characterized with N2-adsorption and desorption, XRD and FT-IR.An optimum condition for immobilization with enzyme immobilization efficiency of about 100% was obtained when the dose of support was greater than 0.45 mg per unit enzyme activity and reaction time exceeded 15 min.HRP immobilized on Al-PILC exhibited good catalytic performance.Aluminum pillared interlayered clay （Al-PILC） calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase （HRP） among several materials investigated. HRP could be immobilized on the Al-PILC via hydrogen bonding between functional groups of support and amino groups of the enzyme. The support and immobilized enzyme were characterized with N2-adsorption and desorption, XRD and FT-IR. An optimum condition for immobilization with enzyme immobilization efficiency of about 100 % was obtained when the dose of support was greater than 0.45 mg per unit enzyme activity and reaction time exceeded 15 min. HRP immobilized on Al-PILC exhibited good catalytic performance.

关 键 词：铝层柱黏土 辣根过氧化物酶 酶固定化 含酚废水处理 

分 类 号：Q814.2[生物学—生物工程] TQ426.97[化学工程]