硝基甲苯对小鼠睾丸生殖细胞DNA的损伤作用  

DNA Damage of Germ Cell in Rats Induced by Nitrotoluenes

作　　者：杨丽[1] 徐镜波[1] 盛连喜[1] 马逊风[1] 孙志伟[2] 石龙[2] 

机构地区：[1]东北师范大学环境科学与工程系,长春130024 [2]吉林大学预防医学院,长春130021

出　　处：《生态毒理学报》2006年第4期303-309,共7页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金资助项目(No.30370262);高等学校博士学科点专项科研基金项目(No.20040200002);国家重点基础研究发展计划(973)项目(No.2004CB418505)

摘　　要：为提供硝基甲苯的环境遗传毒理学依据和建立鱼类生殖细胞的培养方法,采用昆明小鼠睾丸支持细胞/生殖细胞共培养法以及彗星实验,研究了2,4-二硝基甲苯(2,4-DNT)、2,6-二硝基甲苯(2,6-DNT)、对硝基甲苯(4-NT)对小鼠睾丸生殖细胞DNA的损伤作用.结果表明,3种受试硝基甲苯化合物均能够诱导小鼠睾丸生殖细胞DNA单链断裂,而且其受损率与剂量对数具有明显的剂量-效应关系.2,4-DNT、2,6-DNT以及4-NT的各个剂量浓度引起细胞DNA损伤的程度,与对照组相比,均具有显着性差异(p<0.01,p<0.05).受试化合物的毒性顺序为2,6-DNT>2,4-DNT>4-NT,DNA的损伤作用二硝基甲苯大于单硝基甲苯.提示在体外条件下,2,4-DNT、2,6-DNT和4-NT具有生殖毒性,可以引起小鼠睾丸生殖细胞DNA损伤.In order to provide a scientific basis for environmental reproductive toxicology and the culture of germ cell of fish, we study the DNA damage of germ cell of male rat induced by 2,4-DNT, 2,6-DNT and 4-NT using sertoli/germ cell cocultures and comet assay, and also discuss the reproductive toxicology of these three chemicals. The results show that all three nitrotoluene compounds tested （2,4-DNT, 2,6-DNT, 4-NT） could induce DNA strand breaks with significant logarithmic dose-effect relationship. What＇s more, these three chemicals are different in the degree of DNA damage to germ cell of male rat. The sequence is 2,6-DNT〉2,4-DNT〉4-NT, indicating the toxicity of dinitrotoluenes is higher than mononitrotoluene. There is significant （p〈0.01, p〈0.05） difference between the damage rate of these treated groups and that of PBS control, suggesting that 2,4-DNT, 2,6-DNT, 4-NT have geontoxicity and can induce the germ cell DNA strand to break in vitro.

关 键 词：2.4-DNT 2 6-DNT 4-NT 支持细胞 生殖细胞共培养法 彗星实验 DNA损伤 

分 类 号：Q523.03[生物学—生物化学]