碳纳米管导致小鼠肺部急性氧化损伤作用的研究  

Study on Carbon Nanotube Induced Acute Pulmonary Oxidative Damage in Mice

作　　者：李岩[1] 彭光银[1] 何胡军[1] 乔永康[1] 常青[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《生态毒理学报》2006年第4期357-361,共5页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金面上项目(No.30570799);湖北省科技攻关计划项目(No.2005AA307B01)

摘　　要：为了检测未经处理的碳纳米管作为送药载体对肺组织是否会产生毒性作用,将35只雄性昆明小鼠随机分成7组,用腹腔注射进行一次性染尘,6组分别注入0.1、0.2、0.4mg·mL-1的多壁碳纳米管(粒径20￣40nm)或标准碳黑颗粒(粒径<5μm)颗粒悬液1mL,对照组注入等体积生理盐水.染尘7天后对肺组织匀浆中丙二醛(MDA)和谷胱甘肽(GSH)含量进行测定,从而比较二者对肺组织的急性氧化损伤作用.结果表明,不同浓度的碳纳米管染尘组小鼠与对照组小鼠比较,肺组织MDA水平均有显著性升高(p<0.01);但所有标准碳黑组的升高却无统计学意义(p>0.05);0.2、0.4mg·mL-1碳纳米管染尘组与同浓度的标准碳黑染尘组比较,肺组织MDA水平显著升高(p<0.01),0.1mg·mL-1无显著变化.碳纳米管染尘组小鼠肺部GSH含量较对照组均有不同程度的降低,且均有统计学意义(0.1mg·mL-1组p<0.05,0.2mg·mL-1组p<0.01,0.4mg·mL-1组p<0.01).碳黑染尘组也有所降低,但仅0.2mg·mL-1和0.4mg·mL-1组有统计学意义(p<0.05).碳纳米管染尘组小鼠肺部GSH含量与同浓度的标准碳黑组比较,仅0.4mg·mL-1浓度组GSH含量显著降低(p<0.01),其他浓度组无显著变化.结果提示未经处理的碳纳米管作为送药载体对肺将产生一定程度毒性作用,且毒性大于相同浓度的标准碳黑.The present study was conducted to explore whether the acute pulmonary oxidative damage will induced by unfunetion-carbon nanotube（UCNT）（20-40nm） in mice. The animals were divided into 7 testing groups（n=5 for each group） -0.1, 0.2 and 0.4mg·mL^-1 carbon nanotube suspension injected groups, 0.1, 0.2 and 0.4mg·mL^-1 carbon black （CB） suspension injected groups and saline injected group. After 7 days＇ exposure, the contents of malondialdehyde （MDA） and glutathione （GSH） in lungs were measured as indicators of oxidative damages. The results showed that MDA contents in different concentrations of UCNT treated groups were much higher than that of saline group （p 〈0.01 ）, but no significant increase in all CB treated groups （p〉0.05）. Meanwhile, the MDA contents in 0.2 and 0.4mg·mL^-1 UCNT suspension injected groups were also higher than those in the same concentrations of CB treated groups（p〈0.01 ）. As to GSH contents, a significant decrease was found in all the UCNT injected groups （0.1mg·mL^-1 group p〈0.05, 0.2mg·mL^-1 group p〈0.01, 0.4mg·mL^-1 group p〈0.01, compared with control group） and some CB injeced groups （0.2 and 0.4mg·L^-1 groups, p〈 0.05）. After compared the same concentration of UCNT and CB treated groups, it was found that only 0.4mg·mL^-1 UCNT injected group had a significant decrease on GSH contents （p〈0.05, compared with 0.4mg·mL^-1 CB group）. It can be concluded that the acute pulmonary oxidative damage could be induced by UCNT which as medicine vehicle, and it was much severer than that induced by CB.

关 键 词：碳纳米管 标准碳黑 氧化损伤 脂质过氧化反应 

分 类 号：TB838[一般工业技术—摄影技术]