姜黄素对Raji细胞黏附侵袭的调节作用  

Modulatory effect of curcumin on the adhesion and migration of Raji cells

作　　者：吴秋玲[1] 陈燕[1] 张纯[1] 何静[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430000

出　　处：《中国医师杂志》2006年第12期1611-1613,共3页Journal of Chinese Physician

基　　金：国家自然科学基金资助项目(NO30472267)

摘　　要：目的研究姜黄素调节FN/β1整合素和SDF1/CXCR4途径对B淋巴瘤细胞系Raji细胞黏附和迁移的影响,探讨姜黄素抑制淋巴瘤侵袭的机制。方法本实验共分8组,阴性对照、FN、SDF、FN+抗β1整合素抗体、SDF+抗β1整合素抗体、SDF+抗CXCR4抗体、姜黄素+FN、姜黄素+SDF各组,采用黏附、迁移试验检测各组Raji细胞侵袭能力的差异。结果在体外SDF1和FN均能增加Raji细胞黏附、浸润能力,并且此作用能分别被抗β1整合素抗体和抗CXCR4抗体阻断。姜黄素能显著抑制Raji细胞在FN上的黏附以及FN诱导的迁移,并且呈剂量依赖性。低剂量的姜黄素对SDF1诱导的Raji细胞黏附和趋化作用不明显,仅25μmol/L姜黄素能显著抑制SDF1诱导的Raji细胞黏附和趋化。结论FN/β1整合素和SDF1/CXCR4途径在Raji细胞的黏附迁移过程中起重要作用,姜黄素主要通过抑制FN/整合素途径激活来降低Raji细胞的侵袭能力,具有良好的临床应用潜力。Objective To study the effect of SDF1/CXCR4 and FN/β1 integrin modulated by curcumin on the adhesion and migration of B lymphoma Raji cell and to explore the mechanism of curcumin to inhibit the invasiveness of lymphoma. Methods The experiment consisted of eight groups： Control, FN, SDF, FN ＋ anti-β1 integrin, SDF ＋ anti-CXCR4, curcumin ＋ FN, curcumin ＋ SDF, Adhesion assay and migration assay were used to detect the difference of invasiveness among different groups, Results In vitro, SDF1 and FN can increase the adhesion and migration of Raji cells, which can be blocked by β1 integrin antibody and CXCR4 antibody, Curcumin can inhibit the adhesion and migration of Raji to FN in a dose-dependant manner. Low dosage curcumin had minimal effect on the adhesion and migration of Raji induced by SDF except that 25 μmol/L curcumin can inhibit the adhesion ＂and migration of Raji. Conclusion FN/β1 integrin and SDF1/ CXCR4 pathway play important roles in the adhesion and migration of Raji cells. Curcumin can decrease the invasiveness of Raji cells through inhibiting FN/β1 integrin. It has good prospect of clinical application.

关 键 词：姜黄素 淋巴瘤 整合素类 纤连蛋白类 趋化因子 CXC 

分 类 号：R285[医药卫生—中药学]