重组人甲状旁腺激素相关蛋白1-141序列的克隆和表达载体构建  

Cloning and Construction for Expression Vector of Recombinant Human Parathyroid Hormone Related Protein 1-141 Sequence

作　　者：刘新宇[1] 梁东春[2] 左爱军[2] 张镜宇[2] 郭刚[2] 

机构地区：[1]天津医科大学生化教研室,300070 [2]天津市内分泌研究所

出　　处：《天津医药》2006年第12期875-876,I0001,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:39970348);天津市自然科学基金重点项目(项目编号:993804211)

摘　　要：目的:构建人甲状旁腺激素相关蛋白1-141(hPTHrP1-141)基因的表达载体。方法:提取人基因组DNA,PCR扩增PTHrP1-141基因,将其克隆入原核表达载体pQE-30Xa,构建重组表达载体pQE-30Xa/hPTHrP1-141。结果:重组载体pQE-30Xa/hPTHrP1-141经限制性核酸内切酶PvuⅡ酶切鉴定,得到符合预期大小的核酸片段。结论:表达载体pQE-30Xa/hPTHrP1-141构建成功。Objective： To construct expression vector of recombinant human parathyroid hormone related protein 1-141 gene. Methods： Human genomic DNA was extracted. PTHrP1-141 was amplified by PCR and then was inserted into prokaryotic expression vector pQE-30Xa. Then the recombinant plasmid pQE-30Xa/hPTHrPI-141 was constructed. Results： The recombinant plasmid pQE-30Xa/hPTHrP1-141 was identified by restriction endonuclease Pvu II and the fragment which were expected were observed. Conclusion： The recombinant plasmid pQE-30Xa/hPTHrPI-141 was constructed successfully.

关 键 词：甲状旁腺激素相关蛋白质 分子克隆 遗传载体 质粒 遗传重组 人类 

分 类 号：R965.3[医药卫生—药理学]