甲亢大鼠脑甲状腺激素受体mRNA的表达  

Study on the Expression of Thyroid Hormone Receptor mRNA in Hyperthyroid Rat Brain

作　　者：李兰英[1] 刘春蓉[2] 石峰[1] 臧晓怡[1] 刘亚敏[2] 孙云[1] 阚伯红[1] 

机构地区：[1]天津医科大学内分泌研究所,300070 [2]武警医学院病理教研室

出　　处：《天津医药》2006年第12期877-879,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目(项目编号:033605711)

摘　　要：目的:探讨正常大鼠与甲状腺功能亢进(甲亢)大鼠脑甲状腺激素受体(TR)mRNA表达差异。方法:将10只实验大鼠随机分2组,每组5只,荧光定量PCR(FQ-PCR)方法检测甲亢大鼠各脑区甲状腺激素受体mRNA表达变化。结果:与正常大鼠相比,甲亢大鼠大脑、小脑、海马、脑干中TRα1mRNA分别下调43%、35%、52%、49%(P<0.01)。小脑中TRα2mRNA无显著性变化(P>0.05),大脑TRα2mRNA下调34%(P<0.05),海马、脑干中TRα2mRNA分别上调67%(P<0.01)、56%(P<0.05)。小脑中TRβ1mRNA无显著性变化(P>0.05),大脑、海马、脑干中TRβ1mRNA分别下调41%(P<0.01)、50%(P<0.05)、46%(P<0.05)。各脑区TRα1与TRα2mRNA比值均下降(P<0.05)。结论:甲亢时各脑区对高甲状腺激素水平敏感性不一致,甲状腺激素受体mRNA异常表达变化可能参与了甲亢时脑功能障碍的发病机制。Objective： To study the difference of thyroid hormone receptor mRNA expression in normal and hyperthyroid rat brain. Methods： Ten rats were randomly divided into two groups （n=5） including normal control group and hyperthyroid group administrated with thyroid tablets. Thyroid hormone receptor mRNA expressions in various areas of hyperthyroid rat brain were detected by fluorescence quantitation PCR （FQ-PCR）. Results： TRα1 mRNA in hyperthyroid rat decreased 43%, 35%, 52% and 49% in cerebrum, cerebellum, Hippocampus and brain stem respectively compared with control group （P〈 0.01）. TRα2 mRNA remained constant in cerebellum （P 〉 0.05）, decreased 34% in cerebrum （P 〈 0.05） and increased 67% and 56% in hippocampus and brain stem respectively （P 〈 0.01, P 〈 0.05）. TRβ1 mRNA remained constant in cerebellum （P 〉0.05） and decreased 41%, 50% and 46% in cerebrum, hippocampus and brain stem respectively （P 〈 0.01, P 〈 0.05, P 〈 0.05）. TRα1 ： TRα2 ratio decreased in each brain area （P 〈 0.05）. Conclusion： Different brain area has different response to high thyroid hormone level. The abnormal of TR mRNA expression may participate in the pathogenesis of hyperthyroid mental disorder.

关 键 词：甲状腺功能亢进症 受体 甲状腺激素 RNA 信使 脑 大鼠 

分 类 号：R581.2[医药卫生—内分泌]