促性腺激素对小鼠卵泡生长分化因子9表达的影响  

Effects of gonadotropin on the expression of growth differentiation factor 9 and 9B in mouse ovary

作　　者：李春艳[1] 赵跃然[2] 陈子江[1] 赵力新[1] 范秀玲[1] 盛燕[1] 唐蓉[1] 

机构地区：[1]山东大学山东省立医院生殖医学中心,济南250021 [2]山东大学山东省立医院中心实验室,济南250021

出　　处：《中华妇产科杂志》2006年第12期806-809,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的探讨促性腺激素对小鼠卵泡生长分化因子9(GDF-9)及生长分化因子9B(GDF-9B)表达的影响。方法以性成熟小鼠体外培养卵泡和性成熟雌性BALB/c小鼠作为研究模型,(1)体内实验:20只小鼠随机分为两组,A组10只,注射孕马血清促性腺激素(PMSG),每只10IU,B组10只,注射同等体积的生理盐水,24h后取卵巢组织,免疫组化检测GDF-9的表达,原位杂交技术检测GDF-9B的表达,比较两组间GDF-9、GDF-9B的表达差异。(2)体外实验:小鼠卵巢组织经胶原酶消化后收取卵泡58个,随机分为两组进行体外培养,C组32个卵泡,培养液中加入卵泡刺激素(FSH)100IU/L,D组26个卵泡,培养液中不加FSH。C、D组卵泡培养72h后收集卵母细胞,行免疫组化检测,根据细胞着色强度(+^++++)判定GDF-9表达情况。结果(1)免疫组化:共计数370个卵泡,其中A组186个,染色强度为+^++++的卵泡数分别为41(22·0%)、86(46·2%)、42(22·6%)、17(9·1%)个,B组184个,染色强度为+^++++的卵泡数分别为78(42·4%)、79(42·9%)、26(14·1%)、1(0·5%)个,A组较B组卵巢组织GDF-9表达增强,差异有统计学意义(P<0·01)。卵泡培养后共收集卵母细胞58个,C组32个,染色强度为+^+++的卵泡数分别为9(28·1%)、17(53·1%)、6(18·8%)个;D组26个,染色强度为+^+++卵泡数分别为16(61·5%)、8(30·8%)、2(7·7%)个,C组较D组GDF-9的表达增强,差异也有统计学意义(P<0·05)。(2)原位杂交:共计数卵泡362个,其中A组184个,染色强度为+^++++的卵泡数分别为58(31·5%)、85(46·2%)、39(21·2%)、2(1·1%)个,B组178个,染色强度为+^++++的卵泡数分别为71(39·9%)、74(41·6%)、28(15·7%)、5(2·8%)个,A组与B组卵巢组织中GDF-9B的表达强度比较,差异无统计学意义(P>0·05)。结论促性腺激素在体内可增加卵巢组织GDF-9的表达,FSH在体外可增加卵泡GDF-9的表达,促性腺激素不影响GDF-9B的表达。Objectives To study the effects of gonadotropin on the expression of growth differentiation factor-9 （ GDF-9 ） and -9 B （ GDF-9 B ） in mouse ovary. Methods We chose follicles of mature mice cultured in vitro and mature BALB/c mice as our animal models. （ 1 ） In vivo experiment. Twenty mice were divided into two groups （ groups A and B） randomly with ten mice in each group. Each mouse was injected with 10 IU pregnant mare＇s serum gonadotropin in group A and saline of the same volume was given to the other group. Twenty-four hours later we obtained ovarian tissue and immunohistochemistry and in situ hybridization were performed to detect the expression of GDF-9 and GDF-9B. （2）In vitro experiment. Fiftyeight separated follicles were divided into two groups （ groups C and D ） randomly and cultured in vitro. Thirty-two follicles in group C were cultured in medium with follicular stimulating hormone（FSH） for 72 hours while 26 were cultured without FSH. Immunohistochemistry was performed to detect the expression of GDF-9 in both groups. Results We abserved 370 follicles by immunohistochemistry. The weak positive （ ＋ or ＋ ＋ ） rate was 22.0% and 46. 2% while the strong positive （ ＋ ＋ ＋ or ＋ ＋ ＋ ＋ ） rate was 22. 6% and 9. 1% in 186 follicles of group A. The weak positive （ ＋ or ＋ ＋ ） rate in group B was 42. 4% and 42. 9% while the strong positive （＋ ＋ ＋ or ＋ ＋ ＋ ＋） rate was 14. 1% and 0.5%. The expression of GDF-9 was higher in group A than that of group B. The weak positive rate,positive rate and strong positive rate in group C was 28.1% ,53.1% and 18.8% while that in group D was 61.5% ,30. 8% and 7.7%, respectively. The expression of GDF-9 was higher in group C than that of group D. We observed 362 follicles by in situ hybridization. The weak positive （ ＋ or ＋ ＋ ） rate was 31.5% and 46. 2% while the strong positive （＋ ＋ ＋ or ＋ ＋ ＋ ＋） rate was 21.2% and 1.1% in 184 follicles of group A . The weak positive （�

关 键 词：促性腺激素类 胞间信号肽类和蛋白质类 卵巢 

分 类 号：R714[医药卫生—妇产科学]