多重PCR荧光微球检测支气管肺洗液非典型病原体和病毒的临床意义  

Multiplexed PCR Fluorescent Microspheres Detection of Atypical Organism and Virus in Bronchoalveolar Lavage Fluid

作　　者：周一平[1] 陆学东[2] 陈小可[1] 夏利萍[3] 杨来智[2] 彭蕻琳[1] 刘慧[1] 

机构地区：[1]广东医学院附属深圳福田人民医院呼吸科,深圳518033 [2]广东医学院附属深圳福田人民医院检验科,深圳518033 [3]广东医学院附属深圳福田人民医院支气管镜室,深圳518033

出　　处：《实用临床医学（江西）》2006年第12期16-19,共4页Practical Clinical Medicine

摘　　要：目的了解下呼吸道感染患者非典型病原体和病毒感染情况。方法采集92例肺炎和急性支气管炎患者支气管肺洗液(BALF),采用多重PCR技术与LuminexxMAP多重分析技术平台相结合,以荧光微球为载体检测非典型病原体和多种病毒。结果10种(14个亚型)病原体阳性检出率为50%(46/92)。其中病毒阳性检出率为33.69%(31/92),以流感病毒A为主,其占病毒阳性检出的67.74%(21/31);非典型病原体阳性检出率为16.30%(15/92),以MP为多,其占非典型病原体阳性检出的73.33%(11/15)。MP、病毒和细菌混合感染率为13.04%(12/92)。结论流感病毒A和肺炎支原体是本地区社区获得性肺炎和急性气管-支气管炎重要的病原体,并有相互混合感染或与细菌混合感染,临床应予重视。Objective：To investigate the infection rate of atypical organism and virus in patients with community-acquired acute lower respiratory tract infection. Methods： Ninety-two patients with community-acquired pneumonia and acute bronchitis were included in this study. Bronchoalveolar lavage fluids were sampled for detection. Specific multiplexed fluorescent microspheres, multiplex PCR technology and Luminex xMAP （flexible Multi-Analyte Profiling） were combined to detect the DNA or RNA of 10 respiratory pathogens （14 typing） including Adenovirus （ADV）, influenza A virus and Influenza B virus （ FluA , FluB ）, respiratory syncytial virus （RSV）, parainfluenza type 1,2,3,4 virus （ PIV1,PIV2,PIV3, PIV4 ）, Rhinovirus （RhiV）, severe acute respiratory syndrome coronavirus （SARS-Cov）, human metapneumovirus （hMPV）, Mycoplasma pneumoniae （MP）, Chlamydia pneumoniae （CP） and Legionella pneumophilia （LP）. Results： Forty-six of 92 patients （50%） were found of positive for virus or MP or CP. The positive rates were 33.69%（31/92） for viruses and 16.30% （15/92） for atypical organisms. 67.74% （21/31） of the viruses were influenza A virus, 73. 33% （11/15） of the atypical organisms were MP. The mixed infection rate of MP, viruses and bacteria was 13.04% （12/92）. Conclusion： Influenza A virus and MP were the important pathogens in patients with community-acquired pneumonia and acute tracheobronchitis in our community. The pathogens could be mixed with each other or with bacteria to cause infection. The mixed infection of MP, viruses and bacteria should not be ignored.

关 键 词：非典型病原体 病毒 支气管肺泡灌洗液 

分 类 号：R37[医药卫生—病原生物学] R56[医药卫生—基础医学]