海绵来源真菌黄灰青霉Sp-19中的抗肿瘤活性成分研究  

Antitumor components from sponge-derived fungus Penicillium auratiogriseum Sp-19

作　　者：辛志宏[1] 方玉春[1] 朱天骄[1] 段琳[1] 顾谦群[1] 朱伟明[1] 

机构地区：[1]教育部海洋药物重点实验室山东省海洋药物重点实验室中国海洋大学海洋药物与食品研究所

出　　处：《中国海洋药物》2006年第6期1-6,共6页Chinese Journal of Marine Drugs

基　　金：山东省重点基金(No.Z2006C13);中澳国际科技合作计划项目(No.2005DFA30030-4);国家863计划资助项目(2003A624020)

摘　　要：目的对一株来源于羽毛山海绵Mycale plumose的真菌黄灰青霉Penicillium auratiogriseumSp-19发酵产物中的抗肿瘤活性成分进行分离和鉴定。方法采用溶剂萃取、硅胶柱色谱及制备HPLC等分离手段对该菌株发酵产物的活性部位进行了活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB法评价了化合物的抗肿瘤活性。结果与讨论从发酵产物中分离得到5个生物碱类化合物,其结构分别鉴定为fructigenines A(1),去乙酰化fructigenines A(2),fructigeninesB(3),1,4-benzodiazepine-2,5-diones(4)和cyclopenin(5);化合物4和5在3μg.mL-1时对小鼠乳腺癌tsFT210细胞具有强的细胞毒活性。Objective To isolate and identify the antitumor bioaetive components from the fermentation of the fungus Penicillium auratiogriseum Sp-19 derived from sponge Mycale plumose. Methods The antitumor components were isolated by bioassay-guided fraetionation and using solvent extraction, silica gel column chromatography and preparative HPLC. Their structures were established by physicochemical properties and spectral analyses. The eytotoxicities of compounds were evaluated by SRB method. Results and Conclusion Five alkaloid compounds were isolated and identified as fructigenines A （1）, deacetyl fructigenines A （2）, fructigenines B （3）, 1, 4-benzodiazepine-2, 5-diones （4） and cyclopenin （5）, respectively. Compound 4 and 5 showed eytotoxicity at the concentration of 3 μg·mL^-1against tsFT210 cell line.

关 键 词：黄灰青霉菌 羽毛山海绵 次级代谢产物 生物碱 抗肿瘤活性 

分 类 号：R93[医药卫生—生药学] R917[医药卫生—药学]