皱瘤海鞘质量HPLC指纹图谱的研究  

Study on the HPLC fingerprints quality control of ascidian Styela plicata

作　　者：李春荣[1] 曾凡林[1] 邱洁苗[2] 万新祥[1] 

机构地区：[1]南方医科大学药剂研究中心,广东广州510315 [2]南方医科大学南方医院内分泌科,广东广州510515

出　　处：《中国海洋药物》2006年第6期33-37,共5页Chinese Journal of Marine Drugs

基　　金：广东省社会发展计划资助项目(No.2004B30101009)

摘　　要：目的应用HPLC法建立皱瘤海鞘质量HPLC指纹图谱。方法采用Agilent ZORBAX Bonus-RP柱(4.6×250mm,5μm),以0.01%TFA的双蒸水(A)和乙腈(B)为流动相梯度洗脱,检测波长254nm,柱温30℃,记录时间60min,测定10批样品的指纹图谱。结果皱瘤海鞘有效部位有13个共有峰,多数峰都可以达到较好分离,该分析方法具有很好的精密度、重现性和稳定性。结论该方法操作简便、结果可靠,可以对皱瘤海鞘进行鉴定,并为其制剂原料的质量控制提供有效手段。Objective To establish the HPLC fingerprints for the guality control of ascidian Styela plicata. Methods The HPLC fingerprints of ten batches of samples were obtained using ZORBAX Bonus-RP（4.6 ×250mm, 5μm） column with a mix of acetonitrile and water with 0.01%TFA as mobile phase in a gradient mode, the detection wavelength was 254nm and the temperature was 30℃. Results The experiment and analysis were carried out on ten samples, the standard HPLC fingerprint pattern method and 13 characteristic diffraction peaks of Styela plicata were obtained. The proposed method was precise, reproducible and steady. Conclusion This reliable and convenient method can be used for the identification and quality control of Styela plicata.

关 键 词：海鞘 皱瘤海鞘 指纹图谱 高效液相色谱法 

分 类 号：Q959.284[生物学—动物学] R917[医药卫生—药物分析学]