高效液相色谱法测定维生素E粉含量  

Determination of vitamin E in vitamin E powder by HPLC

作　　者：商军[1] 华贤辉[1] 王蓓[1] 虞哲高 陈晓莉 

机构地区：[1]上海市兽药饲料监察所 [2]帝斯曼维生素(上海)有限公司

出　　处：《中国饲料》2006年第24期21-23,28,共4页China Feed

摘　　要：用高效液相色谱法测定饲料添加剂维生素E粉中维生素E含量。采用EclipseXDBC18柱(150mm×4.6mm,5μm),以甲醇-水(98∶2,V∶V)为流动相,流速为1.2mL/min,检测波长为285nm,柱温为25℃。在此色谱条件下,维生素E在0.104￣0.602mg/mL浓度范围内呈良好线性关系,r=0.9998,平均回收率为100.3%,RSD为0.72%。本法操作简便易行,系统适用性良好,适用于饲料添加剂维生素E粉中维生素E含量测定。To establish a method for determination of vitamin E in vitamin E powder for feed additive by HPLC.The separation was performed on Eclipse XDB ODS column （150 mm× 4.6 mm,5 μm） with mobile phase of methanol-water （98：2,V/V） and flow rate was 1.2 mL/min. The determination was done at the column temperature 25℃ and at the wavelength 285 nm.The calibration curve for vitamin E was linear in 0.104 - 0.602 mg/mL （r=0.9998）,the mean recovery was 100.3 % ,RSD=0.72 %（n=9）.The method was simple,rapid and reliable for the determination of vitamin E in vitamin E powder for feed additive.

关 键 词：饲料添加剂 维生素E 高效液相色谱法 

分 类 号：S816.17[农业科学—饲料科学]