彩色马蹄莲欧文氏菌PCR检测  

Detection of Erwinia carotovora subsp.carotovora by PCR

作　　者：王丽花[1] 王继华[1] 杨秀梅[1] 张璐萍[1] 苏艳[1] 

机构地区：[1]云南省农业科学院质标所

出　　处：《植物保护》2006年第6期75-78,共4页Plant Protection

基　　金：云南省科技攻关项目(2004NG08)

摘　　要：采用聚合酶链式反应(PCR)技术检测彩色马蹄莲欧文氏菌纯培养和彩色马蹄莲样品,并用分离培养技术加以验证。结果表明,PCR能特异地检测出所有10个彩色马蹄莲欧文氏菌菌株,并证实了昆明地区侵染彩色马蹄莲的细菌为胡萝卜软腐欧文氏菌(Erwiniacarotovorasubsp.carotovora)。PCR与分离培养技术检测结果基本一致,但总体上PCR检测阳性率稍高于分离培养检测的发病率。接种马铃薯、大白菜24h后接种点处均出现明显软腐症状。该项技术具有更高的灵敏度,适用于彩色马蹄莲种苗的检测和病害流行学研究。Erwinia carotovora subsp, carotovora, which causes soft rot to Zantedeschia aethiopica, was detected by PCR. PCR technique was successful in detecting 10 strains isolated in Kunming, and the strains were identified as E. carotowra subsp, carotowora, The PCR procedure gave similar results to those by biological methods, but more sensitively. The pathogenie fungi could cause soft rot to potato and Chinese cabbage 24 hours after inoculation. It was thus suggested that PCR technique be employed in testing and epidemiological research on Z. aethiopica plants.

关 键 词：彩色马蹄莲 欧文氏菌 PCR 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]