反相高效液相色谱法测定丹栀逍遥片中丹皮酚的含量  

Determination of Paeonol in Danzhi Xiaoyao Tablets by RP-HPLC

作　　者：雷玉萍[1] 李瑞莲[1] 

机构地区：[1]湖南省药品检验所,长沙410001

出　　处：《中国药品标准》2006年第6期47-48,共2页Drug Standards of China

摘　　要：目的:建立丹栀逍遥片中丹皮酚的反相高效液相色谱测定法。方法:采用PolarisC18柱,流动相为甲醇-水(45∶55);检测波长为274nm。结果:丹皮酚的进样量在0.0517μg～0.4653μg范围内与其峰面积有良好的线性关系(r=0.9999),平均加样回收率为98.0%,RSD为1.4%。结论:该方法简便易行、准确可靠,可用于丹栀逍遥片的质量控制。Objective：To establish a RP-HPLC method for content determination of Paeonol in Danzhi Xiaoyao Tablets. Methods： The condition of RP-HPLC was Polaris C18 column,methanol-water（45 ： 55） as mobile phase;the detective wavelength was 274nm. Results ： The calibration cure was linear in the range of 0. 0517 μg-0. 4653 μg of Paeonol （r=0. 9999）. The average recovery and RSD of Puerarin were 98.0% and 1.4%,respectively. Conclusion：The method is simple,accurate and reliable and can be used for the quality control of Danzhi Xiaoyao Tablets.

关 键 词：丹栀逍遥片 丹皮酚 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]