Effects of medium nutrition on cell growth and isocamptothecin A and B production by suspension cell culture of Camptotheca acuminata  

作　　者：张冬艳 Yu Fang Bai Fengwu An Lijia 

机构地区：[1]Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116023, P.R. China

出　　处：《High Technology Letters》2006年第4期446-448,共3页高技术通讯（英文版）

基　　金：Supported by the National Natural Science Foundation of China （No. 20176058）.

摘　　要：The effects of initial sucrose concentration, nitrate to ammonium ratio, total N concentration and phosphate concentration in medium on cell growth and isocamptothecin A and B synthesis by suspension call culture of Camptotheca acuminata were investigated in 250 mL shake flasks. 30 g L^-1 sucrose concentration was beneficial to secondary metabolites synthesis. The cell growth and metabolites synthesis were also affected by the ratio of NO3^-/NH4^＋ , and nitrate was tavourable for cell growth. The maximum dry weight was achieved when nitrate was used as the sole N souree. The effect of total initial N on the cell cultures was also investigated with NO3^-/NH4^＋ ratio of 1 ： 2. The final dry cell weight was similar throughout culture period and 50 mM initial N was favourable for secondary metabolite synthesis. 50 mM initial phosphate concentration facilitated both cell growth and secondary metabolites synthesis.

关 键 词：Camptotheca acuminata isocamptothecin A and B plant cell culture 

分 类 号：Q943.1[生物学—植物学]