8-氮杂腺嘌呤及其金属配合物与DNA的作用机理  

The Action Mechanism of 8-Azaadenine and Its Metal Complexes with DNA

作　　者：席小莉[1] 杨曼曼[1] 杨频[1] 

机构地区：[1]山西大学化学化工学院分子科学研究所化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《光谱学与光谱分析》2006年第12期2272-2275,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20601018);山西省自然科学基金项目资助

摘　　要：8-氮杂腺嘌呤是抗代谢类药物,临床上广泛用于治疗增殖很快的肿瘤。文章以溴化乙啶为探针,通过紫外光谱,荧光光谱,研究了8-氮杂腺嘌呤及其与Co2+和Cu2+离子形成的配合物同DNA的作用。结果发现:在pH 9.86时,8-氮杂腺嘌呤可与Co2+形成稳定配合物,此配合物与DNA之间静电作用较强、嵌插作用较弱;在pH 7.4时,8-氮杂腺嘌呤可与Cu2+形成稳定配合物,其与DNA之间主要以嵌插作用为主、静电作用为辅;在pH 2.85时,8-氮杂腺嘌呤-Cu2+配合物与DNA之间嵌插作用较强、静电作用较弱;在pH 7.4时,8-氮杂腺嘌呤几乎不影响EB和DNA之间的嵌入结合方式,它与DNA为静电方式结合。这些结果,可为合理改善药效,降低抗癌药物毒性和设计新药提供理论依据。The mechanism of the interactions of 8-azaadenine and its Co^2＋ and Cu^2＋ complexes with DNA was studied by UV and fluorescence spectra using ethidium bromide（EB） as the probe molecule. When pH 9.86, the action of 8-azaadenine-Co^2＋ with DNA was electrostatic effect and certain kind of intercalation action, when pH 7.4, the action of 8-azaadenine-Cu^2＋ with DNA was intercalation action and certain kind of electrostatic effect, when pH 2. 85, the action was intercalation action and certain kind of electrostatic effect. But when pH 7.4, 8-azaadenine hardly influenced the intercalation action of EB and DNA, and the action of 8-azaadenine and DNA was electrostatic action.

关 键 词：8-氮杂腺嘌呤 金属配合物 DNA Scatchard图 

分 类 号：O614.8[理学—无机化学]