鳞翅目昆虫细胞抗菌肽的诱导、筛选及抗菌活性的研究  

Induction,selection and antibacterial activity studies of the antibacterial peptides from the cultured insect cell lines

作　　者：彭蓉[1] 杨忠[2] 刘凯于[1] 姚汉超[1] 杨红[1] 崔艳芳[3] 洪华珠[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079 [2]华东理工大学生物工程学院,上海200237 [3]华中师范大学化学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2006年第2期240-243,共4页Journal of Central China Normal University：Natural Sciences

基　　金："973"重大基础研究前期专项(2002CCA02400);国家自然科学基金项目(20475019);上海市科技启明星计划

摘　　要：选取3种离体培养的鳞翅目昆虫细胞系,采用热灭活的大肠杆菌诱导的方法,诱导其产生抗菌肽,并进行抗菌活性检测、诱导动力学研究及Tricine SDS-聚丙烯酰胺凝胶电泳检测分析.结果筛选到1株诱导抗菌活性较高的昆虫细胞系———粉纹夜蛾BTI-Tn-5B1细胞系,并发现该粉纹夜蛾5B1细胞在诱导后16 h产生了1个分子量大约为8 000的抗菌肽,抗菌活性检测表明其对金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coliK12D31)、德氏卑沙门氏菌(Salmonella derby)均有不同程度的抑制作用,其中特别是对革兰氏阴性菌———大肠杆菌K12D31和德氏卑沙门氏菌有较强的抑菌活性.Spodoptera exigua cell line, Spodoptera litura cell line and Trichoplusia ni BTI-Tn-5B1 cell line,these three cultured cells in vitro from Lepidoptera, were stimulated with heat-killed Escherichia coli DH5α, antibacterial activity assays showed that the antibacterial activity of the induced BTI-Tn-5B1 cell line was the highest, and the antibacterial activity increased gradually to the highest in 16 hours after stimulation. Tricine SDS-PAGE experiment found that a new antibacterial peptide, with a molecular weight of about 8 000, was preferentially induced in BTI-Tn-5B1 cells in 16 hours after stimulation. Results of antibacterial activity assay indicated that it had inhibitory activities against some Gram-positive and Gram-negative bacteria, especially showing strong activities to Escherichia coil K12D31 and Salmonella derby.

关 键 词：抗菌肽 BTI-Tn-5B1细胞系 Tricine SDS-聚丙烯酰胺凝胶电泳 抗菌活性 

分 类 号：Q966[生物学—昆虫学]