葡萄糖氧化酶基因植物表达载体的构建  

Construction of Glucose Oxidase Gene in Plant Expression Binary Vector

作　　者：孙瑞芬[1] 安玉麟[1] 张鹤龄[2] 兰海英[1] 王静[3] 程继东[3] 刘力萍[1] 

机构地区：[1]内蒙古农牧业科学院生物技术中心,内蒙古呼和浩特010031 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [3]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《华北农学报》2006年第6期11-15,共5页Acta Agriculturae Boreali-Sinica

基　　金：内蒙古自治区优秀学科带头人项目(20050802)

摘　　要：为进一步开展转基因研究创造条件,用限制性内切酶将葡萄糖氧化酶(GOD)基因从pMD/GO载体上切下,定向连接到植物表达载体pROKⅡ的CaMV35S启动子下游和NOS终止子上游,成功地构建了GOD基因植物表达载体pROK/GO。利用冻融法将此表达载体导入根癌农杆菌LBA4404中,提取转化质粒,经PCR和酶切鉴定表明,GOD基因植物表达双元载体构建成功。This is the preparation for genetic transformation. Glucose oxidase gene was cut from pMD/GO with restraction enzymes. A plant expression vector pROK/GO was constructed. The fragment of Glucose oxidase gene in pROKⅡ was directively ligated downstream to CaMV35S promotor and upstream to NOS terminator. The plant expression vector was transformed into A. tumefaciens LBA4404 by the freeze-thaw method. The binary expression plasmid was further identified by PCR and restriction enzymes digestion. The results showed that the plant expression binary vector pROK./GO was constructed successfully.

关 键 词：葡萄糖氧化酶基因 植物表达载体 

分 类 号：Q782[生物学—分子生物学]