萝卜花药培养技术研究  

A Study of Anther Culture of Radish(Raphanus sativus L.)

作　　者：张丽[1] 赵秋菊[2] 付传翠[3] 白小娟[4] 

机构地区：[1]国家蔬菜工程技术研究中心,北京100097 [2]北京市农林科学院,北京100097 [3]莱阳农学院园艺系,山东青岛266109 [4]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《华北农学报》2006年第6期55-58,共4页Acta Agriculturae Boreali-Sinica

基　　金：北京市科技新星资助项目(B类;H020821470130)

摘　　要：通过单倍体育种途径可快速获得纯合材料,增加有益性状的选择几率,加快育种进程。花药培养是获得单倍体的有效途径之一,已在十字花科芸薹属作物上取得巨大成功。但关于萝卜花药培养的报道极少。以15个不同类型的萝卜品种为试材,研究了花药离体培养中胚状体的诱导及再生。结果表明:基因型是限制花药培养成胚的关键因素,15个品种中,有4个品种获得了胚状体及再生苗。同时植株生长条件也对胚状体的形成产生影响。采用流式细胞仪(FCM)鉴定了再生株的倍性,再生株群体的倍性组成比较复杂,以二倍体为主。Homogenous lines can be obtained in a very short time by haploidy breeding method such as microspore culture and anther culture. Techniques for anther culture has been developed and widely used for most Brassica species, but not for radish（ Raphanus sativus L. ）. Anther of 15 genotypes of radish have been cultured in vitro and induced to undergo embryogenesis and plant formation. Of 15 genotypes evaluated, four produced embyos ranging from 21.3 to 2.0. The genotype was the main factor to influence the embyogenesis. In addition the growing condition of donor plants also influenced the embyogenesis. FCM analysis of ploidy level of anther-derived plants was made.

关 键 词：萝卜 花药培养 单倍体育种 

分 类 号：S631.1[农业科学—蔬菜学]