兔急性心肌缺血脱氢酶电镜-细胞化学变化的动态观察  

Electron Microscopical Cytochemical Dynamic Obsensation of Dehydrogenases on Experimental Rabbit's Acute Myocardial Ischemia

作　　者：张国华[1] 李德祥[1] 

机构地区：[1]中国医科大学法医病理教研室

出　　处：《法医学杂志》1996年第4期195-196,共2页Journal of Forensic Medicine

摘　　要：为了研究急性心肌缺血脱氢酶变化的规律性，本文应用电镜酶细胞化学技术，通过结扎５０只家兔冠状动脉左前降支，观察心肌缺血后０．２５、０．５、１、１．５、２、４、６、１２、２４、３６、７２小时ＳＤＨ和ＬＤＨ的变化。结果显示：心肌缺血０．５小时后，ＳＤＨ和ＬＤＨ活性开始下降。同时伴有致密体的形成和超微结构的改变。随着缺血时间的延长，酶活性下降更加明显，最后消失。The purpose of this study was to investigate the changes of dehydrogenases in acute myocardia ischemia in rabbits.The succinate dehydrogenase(SDH) and lactate dehydrogenase(LDH) were observed by using electron microscopical technique at the time of 0.25.0.5,1.1.5,2,4,6,12,24,36 and 72 hour respectively aller the ligation of the left anterior descending coronary artery.The results showed that SDH and LDH activity began to decrease in group of coronary occlusion for 0.5 hours.At the same time,amorphous dese body in the mitochondria could be seen and its ultrastructural changes occured.With the prolongation of myocardial ischdria.the decreases of enzymatic activity were obvious.At last,enzymatic granules of SDH and LDH couldn't be found

关 键 词：急性 心肌缺血 琥珀酸脱氢酶 乳酸脱氢酶 

分 类 号：R542.2[医药卫生—心血管疾病] R541.4[医药卫生—内科学]