新生大鼠心肌细胞培养方法的改进  

Isolation and culture of cardiomyocytes in neonatal rat

作　　者：郭军[1] 鲍翠玉[1] 林国生[2] 杨波[2] 

机构地区：[1]咸宁学院心血管疾病研究所,湖北咸宁市437100 [2]武汉大学人民医院心内科,湖北武汉430060

出　　处：《心血管康复医学杂志》2006年第6期538-540,共3页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：湖北省教育厅资助项目(2004Q001)

摘　　要：目的：建立便捷，成熟的新生大鼠心肌细胞体外培养方法。方法：本实验对常用的培养方法加以改进．采用胰蛋白酶及Ⅰ型、Ⅱ型胶原酶共同消化法，分离新生大鼠心肌细胞，并对体外培养的心肌细胞的存活率、活力及纯度进行鉴定。结果：分离的心肌细胞存活率为96％，培养3d后有60-80％的细胞开始搏动，搏动频率平均50次／min，心肌细胞纯度在75％以上。结论：本实验建立的新生大鼠心肌细胞体外培养方法是成熟，可靠的。Objective： To establish a convenient and mature method of isolation and culture of cardiomyocytes in neonatal rat. Methods： Cardiomyocytes of neonatal rat were isolated in trypsin and collagenase. Then the livability, energy and the purity of cardiomyocytes were identified. Results： The livability of cardiomyocytes was 96 % ; 60%- 80% beating cardiomyocytes were observed and the beating frequency of cells was 50 beats/min after 3 days. The purity of cardiomyocytes was 75 %. Conclusion： This method of isolation and culture of cardiomyocytes in neonatal rat was mature.

关 键 词：心肌 大鼠 细胞培养 

分 类 号：R446.09[医药卫生—诊断学]